Evidence map›Paper›PMID 38570716›Full record

ReviewNature2024

Bridging structural and cell biology with cryo-electron microscopy.

Eva Nogales, Julia Mahamid

Open access · greenAbstract readReview
In one paragraph

Review in Nature, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.

0numbers the graph read from it
0cells of the map it votes in
101citing papers in PubMed
86.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

101 citing papers in PubMed, 166 citations in OpenAlex.

  1. Visualization of membrane-stabilized SorCS2Journal of structural biology: X · 2026
    Article
  2. Article
  3. The molecular basis of transcription initiation by RNA polymerase II.Nature reviews. Molecular cell biology · 2026
    Review
  4. eLife · 2026
    Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. LowDoseWizard: rapid and standardized setup of low-dose cryo-TEM imaging in SerialEM.Acta crystallographica. Section D, Structural biology · 2026
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Review
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article

41 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 3 countries.

Eva NogalesMolecular and Cell Biology Department, Institute for Quantitative Biomedicine, University of California, Berkeley, CA, USA. enogales@lbl.gov.ORCID 0000-0001-9816-3681
Julia MahamidStructural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. julia.mahamid@embl.de.ORCID 0000-0001-6968-041X
European Bioinformatics Institute · GBQB3 · US

Funding

Structural studies of function and regulation of microtubules and transcriptional gene expression machineryR35GM127018 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Eva Nogales · 2018 to 2026
$4.3M
NIGMS NIH HHS R35 GM127018
6 · The paper itself

Abstract

Most life scientists would agree that understanding how cellular processes work requires structural knowledge about the macromolecules involved. For example, deciphering the double-helical nature of DNA revealed essential aspects of how genetic information is stored, copied and repaired. Yet, being reductionist in nature, structural biology requires the purification of large amounts of macromolecules, often trimmed off larger functional units. The advent of cryogenic electron microscopy (cryo-EM) greatly facilitated the study of large, functional complexes and generally of samples that are hard to express, purify and/or crystallize. Nevertheless, cryo-EM still requires purification and thus visualization outside of the natural context in which macromolecules operate and coexist. Conversely, cell biologists have been imaging cells using a number of fast-evolving techniques that keep expanding their spatial and temporal reach, but always far from the resolution at which chemistry can be understood. Thus, structural and cell biology provide complementary, yet unconnected visions of the inner workings of cells. Here we discuss how the interplay between cryo-EM and cryo-electron tomography, as a connecting bridge to visualize macromolecules in situ, holds great promise to create comprehensive structural depictions of macromolecules as they interact in complex mixtures or, ultimately, inside the cell itself.

Indexed as

Cell BiologyCellsCryoelectron MicroscopyElectron Microscope TomographyHumansMacromolecular SubstancesMacromolecular Substances

Identifiers

PMID38570716
PMCPMC11211576
OpenAlexW4393853704

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.