Evidence map›Paper›PMID 38594674›Full record

ArticleCell communication and signaling : CCS2024

Proteomic studies of VEGFR2 in human placentas reveal protein associations with preeclampsia, diabetes, gravidity, and labor.

Shannon J Ho, Dale Chaput, Rachel G Sinkey, Amanda H Garces, Erika P New, Maja Okuka, Peng Sang, Sefa Arlier, Nihan Semerci, Thora S Steffensen and 8 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell communication and signaling : CCS, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 5 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 3 institutions in 1 country.

Shannon J Ho *Department of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA.
Dale Chaput *Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL, USA.
Rachel G SinkeyDepartment of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA.
Amanda H GarcesLisa Muma Weitz Microscopy Laboratory, University of South Florida, Tampa, FL, USA.
Erika P NewDepartment of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA.
Maja OkukaDepartment of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA.
Peng SangDepartment of Chemistry, University of South Florida, Tampa, FL, USA.
Sefa ArlierDepartment of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA.
Nihan SemerciDepartment of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA.
Thora S SteffensenDepartment of Pathology, Tampa General Hospital, Tampa, FL, USA.
Thomas J RutherfordDepartment of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA.
Angel E AlsinaTransplant Surgery Center, Tampa General Hospital, Tampa, FL, USA.
Jianfeng CaiDepartment of Chemistry, University of South Florida, Tampa, FL, USA.
Matthew L AndersonDepartment of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA.
Ronald R MagnessDepartment of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA.
Vladimir N UverskyDepartment of Molecular Medicine, University of South Florida, Tampa, FL, USA.
Derek A T CummingsDepartment of Biology and Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA.
John C M TsibrisDepartment of Obstetrics and Gynecology, University of South Florida, Tampa, FL, USA. tsibris@usf.edu.
University of South Florida · USTampa General Hospital · USUniversity of Florida · US

Funding

TRANSLATIONAL RESEARCHP30CA076292 · NCI · UNIVERSITY OF SOUTH FLORIDA · PI John L. Cleveland · 1998 to 2026
$93.5M
The ACHIEVE Trial: Achieving longer gestation in preeclampsia via antihypertensive therapy.K23HL159331 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Rachel G Sinkey · 2022 to 2026
$884k
NCI NIH HHS P30 CA076292NHLBI NIH HHS K23 HL159331
6 · The paper itself

Abstract

VEGFR2 (Vascular endothelial growth factor receptor 2) is a central regulator of placental angiogenesis. The study of the VEGFR2 proteome of chorionic villi at term revealed its partners MDMX (Double minute 4 protein) and PICALM (Phosphatidylinositol-binding clathrin assembly protein). Subsequently, the oxytocin receptor (OT-R) and vasopressin V1aR receptor were detected in MDMX and PICALM immunoprecipitations. Immunogold electron microscopy showed VEGFR2 on endothelial cell (EC) nuclei, mitochondria, and Hofbauer cells (HC), tissue-resident macrophages of the placenta. MDMX, PICALM, and V1aR were located on EC plasma membranes, nuclei, and HC nuclei. Unexpectedly, PICALM and OT-R were detected on EC projections into the fetal lumen and OT-R on 20-150 nm clusters therein, prompting the hypothesis that placental exosomes transport OT-R to the fetus and across the blood-brain barrier. Insights on gestational complications were gained by univariable and multivariable regression analyses associating preeclampsia with lower MDMX protein levels in membrane extracts of chorionic villi, and lower MDMX, PICALM, OT-R, and V1aR with spontaneous vaginal deliveries compared to cesarean deliveries before the onset of labor. We found select associations between higher MDMX, PICALM, OT-R protein levels and either gravidity, diabetes, BMI, maternal age, or neonatal weight, and correlations only between PICALM-OT-R (p < 2.7 × 10

Indexed as

Diabetes MellitusPre-EclampsiaFemaleGravidityHumansInfant, NewbornOxytocinPlacentaPregnancyProteomicsReceptors, OxytocinVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2KDR protein, humanOxytocinReceptors, OxytocinVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2Hofbauer cellsMDMXOxytocin receptorPICALMPlacentaProteomicsTRIM21TRPM7Vasopressin receptor V1aRVEGFR2

Identifiers

PMID38594674
PMCPMC11003095
OpenAlexW4394619284

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.