Evidence map›Paper›PMID 38621646›Full record

ArticleBiomedical journal2025

Hepatocyte nuclear factor 4 located in different developmental stages of Schistosoma japonicum and involved in important metabolic pathways.

Kaijuan Wu, Shuaiqin Huang, Yiming Zhao, Abdulrahim Umar, Hao Chen, Zheng Yu, Jing Huang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Kaijuan WuDepartment of Parasitology, School of Basic Medical Science, Central South University, Changsha, Hunan, China; China-Africa Research Center of Infectious Diseases, Central South University, Changsha, Hunan, China.
Shuaiqin HuangDepartment of Parasitology, School of Basic Medical Science, Central South University, Changsha, Hunan, China; China-Africa Research Center of Infectious Diseases, Central South University, Changsha, Hunan, China.
Yiming ZhaoDepartment of Microbiology, School of Basic Medical Science, Central South University, Changsha, Hunan, China; China-Africa Research Center of Infectious Diseases, Central South University, Changsha, Hunan, China.
Abdulrahim UmarDepartment of Microbiology, School of Basic Medical Science, Central South University, Changsha, Hunan, China.
Hao ChenDepartment of Parasitology, School of Basic Medical Science, Central South University, Changsha, Hunan, China; China-Africa Research Center of Infectious Diseases, Central South University, Changsha, Hunan, China.
Zheng YuDepartment of Microbiology, School of Basic Medical Science, Central South University, Changsha, Hunan, China; China-Africa Research Center of Infectious Diseases, Central South University, Changsha, Hunan, China.
Jing HuangDepartment of Parasitology, School of Basic Medical Science, Central South University, Changsha, Hunan, China; China-Africa Research Center of Infectious Diseases, Central South University, Changsha, Hunan, China. Electronic address: jing_huang@csu.edu.cn.
Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNuclear receptors (NRs) are vital for regulating gene expression in organisms. Hepatocyte nuclear factor 4 (HNF4), a class of NRs, participates in blood feeding and intestinal maintenance in schistosomes. However, there are limited researches on the molecular and functional characterization of HNF4 in Schistosoma japonicum (S. japonicum).

methodsHighly specific polyclonal antibodies were generated to analyze the expression and tissue localization of S. japonicum HNF4 (SjHNF4). The potential biological functions of SjHNF4 were characterized by transcriptome and pull-down analyses. Subsequently, enrichment analysis was performed to identify the specific signaling pathways linked to SjHNF4.

resultsThe SjHNF4 protein was expressed heterologously and purified successfully. High purity and high potency polyclonal antibodies were further prepared. The expression of SjHNF4 was higher in female compared to male worms at both transcriptional and protein levels. Female worms expressed SjHNF4 in their perithecium, reproductive system, and certain parts of the intestinal tissues. SjHNF4 was also detected in the perithecium of male worms, as well as in the head, body of cercariae, and eggs. Furthermore, our findings highlighted the potential role of SjHNF4 in blood feeding and its interaction with crucial pathways such as glucose metabolism, lipid metabolism, and nucleotide metabolism.

conclusionsThis study shed light on the localization of SjHNF4 in different life stages of S. japonicum, particularly associated with the female schistosomes. A strong correlation was observed between SjHNF4 and essential metabolic pathways. These findings laid a solid groundwork for the research on the relationship between NRs and schistosomes.

Indexed as

Hepatocyte Nuclear Factor 4Metabolic Networks and PathwaysSchistosoma japonicumAnimalsFemaleMaleSchistosomiasis japonicaSignal TransductionHepatocyte Nuclear Factor 4Hepatocyte nuclear factor 4Metabolic pathwaysPolyclonal antibodiesSchistosoma japonicumTissue localizationTranscriptome analysis

Identifiers

PMID38621646
PMCPMC12013132
OpenAlexW4394847012

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.