Evidence mapPaperPMID 38622665Full record

ArticleClinical epigenetics2024

DNMT1/miR-152-3p/SOS1 signaling axis promotes self-renewal and tumor growth of cancer stem-like cells derived from non-small cell lung cancer.

Qing Yuan, Rubo Wang, Xiang Li, Fei Sun, Jiazhi Lin, Zhimin Fu, Jiansong Zhang

Open access · goldAbstract read
In one paragraph

Article in Clinical epigenetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Qing Yuan *Department of Preclinical Medicine, Medical College, Hunan Normal University, Changsha, 410013, China.
Rubo Wang *Department of Pathology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, China.
Xiang LiDepartment of Preclinical Medicine, Medical College, Hunan Normal University, Changsha, 410013, China.
Fei SunDepartment of Preclinical Medicine, Medical College, Hunan Normal University, Changsha, 410013, China.
Jiazhi LinDepartment of Gynaecology and Obstetrics, Shenshan Medical Center, Memorial Hospital of Sun Yat-sen University, Shanwei, 516500, Guangdong, China.
Zhimin FuDepartment of Thoracic Surgery, The Tenth Affiliated Dongguan Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, 523000, China. fzmjinan2006@126.com.
Jiansong ZhangDepartment of Preclinical Medicine, Medical College, Hunan Normal University, Changsha, 410013, China. 14928@hunnu.edu.cn.
Hunan Normal University · CNCentral South University · CNDongguan People’s Hospital · CNSun Yat-sen Memorial Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCSLCs(Cancer stem cell-like cells), which are central to tumorigenesis, are intrinsically influenced by epigenetic modifications. This study aimed to elucidate the underlying mechanism involving the DNMT1/miR-152-3p/SOS1 axis in regulating the self-renewal and tumor growth of LCSLCs (lung cancer stem-like cells). MATERIALS AND

methodsTarget genes of miR-152-3p were predicted using TargetScan Human 8.0. Self-renewal and tumor growth of LCSLC were compared in suspension-cultured non-small cell lung cancer (NSCLC) cell lines H460 and A549 cell-derived globe cells. Functional effects of the DNMT1/miR-152-3p/SOS1 axis were assessed through gain-of-function experiments in vitro and in vivo. Additionally, luciferase reporter assays were employed to analyze the interaction among DNMT1, miR-152-3p, and SOS1.

resultsOur findings highlight a negative interaction between DNMT1 and miR-152-3p, resulting in reduced miR-152-3p level. This, in turn, leads to the alleviation of the inhibitory effect of miR-152-3p on the target gene SOS1, ultimately activating SOS1 and playing an essential role in self-renewal and tumor growth of LCSLC. However, the alteration of SOS1 does not affect DNMT1/miR-152-3p regulation. Therefore, it is reasonable to infer that the DNMT1/miR-152-3p negative feedback loop critically sustains self-renewal and tumor growth of LCSLC through SOS1.

conclusionsThis study reveals a novel mechanism underpinning self-renewal and tumor growth of CSLC (cancer stem cell) in NSCLC and identifies potential therapeutic targets for NSCLC treatment.

Indexed as

Carcinoma, Non-Small-Cell LungLung NeoplasmsMicroRNAsCell Line, TumorCell MovementCell ProliferationDNA (Cytosine-5-)-Methyltransferase 1DNA MethylationGene Expression Regulation, NeoplasticHumansNeoplastic Stem CellsSOS1 ProteinDNA (Cytosine-5-)-Methyltransferase 1DNMT1 protein, humanMicroRNAsMIRN152 microRNA, humanSOS1 ProteinSOS1 protein, humanCancer stem cellsDNA Methyltransferase 1miR-152-3pNon-small cell lung cancerSOS1

Identifiers

PMID38622665
PMCPMC11020669
OpenAlexW4394805488

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.