Evidence map›Paper›PMID 38627162›Full record

ArticleAtherosclerosis2024

Stromal cell-derived factor-1 alpha improves cardiac function in a novel diet-induced coronary atherosclerosis model, the SR-B1ΔCT/LDLR KO mouse.

Danielle M Mullis, Amanda Padilla-Lopez, Hanjay Wang, Yuanjia Zhu, Stefan Elde, Spencer A Bonham, Shin Yajima, Olivier N Kocher, Monty Krieger, Y Joseph Woo

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Atherosclerosis, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Danielle M MullisDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA, USA.
Amanda Padilla-LopezDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA, USA.
Hanjay WangDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA, USA.
Yuanjia ZhuDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA, USA; Department of Bioengineering, Stanford University, Stanford, CA, USA.
Stefan EldeDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA, USA.
Spencer A BonhamDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA, USA.
Shin YajimaDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA, USA.
Olivier N KocherDepartment of Pathology, Beth Israel Hospital, Harvard Medical School, Boston, MA, USA.
Monty KriegerDepartment of Biology, Massachusetts Institute of Technology, MA, USA.
Y Joseph WooDepartment of Cardiothoracic Surgery, Stanford University, Stanford, CA, USA; Department of Bioengineering, Stanford University, Stanford, CA, USA. Electronic address: joswoo@stanford.edu.
Stanford University · USHarvard University · USMassachusetts Institute of Technology · US

Funding

ANGIOGENIC TISSUE ENGINEERING TO LIMIT POST-INFARCTION VENTRICULAR REMODELINGR01HL089315 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI WOO, Y JOSEPH · 2008 to 2023
$6.4M
Canonical & non-canonical regulation of the HDL receptor by PDZK1's PDZ domainsR01HL127174 · NHLBI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI KRIEGER, MONTY · 2016 to 2019
$1.9M
Temporospatial Single-Cell Characterization of Angiogenesis and Myocardial Regeneration in Small and Large MammalsF32HL170741 · NHLBI · STANFORD UNIVERSITY · PI ELDE, STEFAN · 2023 to 2024
$160k
NHLBI NIH HHS F32 HL170741NHLBI NIH HHS R01 HL089315NHLBI NIH HHS R01 HL127174
6 · The paper itself

Abstract

BACKGROUND AND

aimsThere are a limited number of pharmacologic therapies for coronary artery disease, and few rodent models of occlusive coronary atherosclerosis and consequent myocardial infarction with which one can rapidly test new therapeutic approaches. Here, we characterize a novel, fertile, and easy-to-use HDL receptor (SR-B1)-based model of atherogenic diet-inducible, fatal coronary atherosclerosis, the SR-B1ΔCT/LDLR KO mouse. Additionally, we test intramyocardial injection of Stromal Cell-Derived Factor-1 alpha (SDF-1α), a potent angiogenic cytokine, as a possible therapy to rescue cardiac function in this mouse.

methodsSR-B1ΔCT/LDLR KO mice were fed the Paigen diet or standard chow diet, and we determined the effects of the diets on cardiac function, histology, and survival. After two weeks of feeding either the Paigen diet (n = 24) or standard chow diet (n = 20), the mice received an intramyocardial injection of either SDF-1α or phosphate buffered saline (PBS). Cardiac function and angiogenesis were assessed two weeks later.

resultsWhen six-week-old mice were fed the Paigen diet, they began to die as early as 19 days later and 50% had died by 38 days. None of the mice maintained on the standard chow diet died by day 72. Hearts from mice on the Paigen diet showed evidence of cardiomegaly, myocardial infarction, and occlusive coronary artery disease. For the five mice that survived until day 28 that underwent an intramyocardial injection of PBS on day 15, the average ejection fraction (EF) decreased significantly from day 14 (the day before injection, 52.1 ± 4.3%) to day 28 (13 days after the injection, 30.6 ± 6.8%) (paired t-test, n = 5, p = 0.0008). Of the 11 mice fed the Paigen diet and injected with SDF-1α on day 15, 8 (72.7%) survived to day 28. The average EF for these 8 mice increased significantly from 48.2 ± 7.2% on day 14 to63.6 ± 6.9% on day 28 (Paired t-test, n = 8, p = 0.003).

conclusionsThis new mouse model and treatment with the promising angiogenic cytokine SDF-1α may lead to new therapeutic approaches for ischemic heart disease.

Indexed as

Chemokine CXCL12Coronary Artery DiseaseDisease Models, AnimalMice, KnockoutReceptors, LDLScavenger Receptors, Class BAnimalsDiet, AtherogenicDiet, High-FatMaleMiceMice, Inbred C57BLMyocardiumNeovascularization, PhysiologicVentricular Function, LeftChemokine CXCL12Cxcl12 protein, mouseReceptors, LDLScarb1 protein, mouseScavenger Receptors, Class BAngiogenesisCoronary atherosclerosisHeartMyocardial infarctionStromal cell-derived factor-1 alpha

Identifiers

PMID38627162
PMCPMC11254567
OpenAlexW4393075875

What Socratic holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.