Evidence map›Paper›PMID 38634010›Full record

ArticleEFSA journal. European Food Safety Authority2024

Technical Note on the quality of DNA sequencing for the molecular characterisation of genetically modified plants.

European Food Safety Authority (EFSA), Adrián César-Razquin, Josep Casacuberta, Tamas Dalmay, Silvia Federici, Sara Jacchia, Dafni Maria Kagkli, Simon Moxon, Nikoletta Papadopoulou

Open access · diamondAbstract read
In one paragraph

Article in EFSA journal. European Food Safety Authority, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
4.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Questions and Consequences of Omics in Genetically Engineered Crop Regulation.Plant-environment interactions (Hoboken, N.J.) · 2025
    Review
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

European Food Safety Authority (EFSA)
Adrián César-Razquin
Josep Casacuberta
Tamas Dalmay
Silvia Federici
Sara Jacchia
Dafni Maria Kagkli
Simon Moxon
Nikoletta Papadopoulou

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As part of the risk assessment (RA) requirements for genetically modified (GM) plants, according to Regulation (EU) No 503/2013 and the EFSA guidance on the RA of food and feed from GM plants (EFSA GMO Panel 2011), applicants need to perform a molecular characterisation of the DNA sequences inserted in the GM plant genome. This Technical Note to the applicants puts together requirements and recommendations for the quality assessment of the methodology, analysis and reporting when DNA sequencing is used for the molecular characterisation of GM plants. In particular, it applies to the use of Sanger sequencing and next-generation sequencing for the characterisation of the inserted genetic material and its flanking regions at each insertion site, the determination of the copy number of all detectable inserts and the analysis of the genetic stability of the inserts. This updated document replaces the EFSA 2018 Technical Note and reflects the current knowledge in scientific-technical methods for generating and verifying, in a standardised manner, DNA sequencing data in the context of RA of GM plants. It does not take into consideration the verification and validation of the detection method which remains under the remit of the Joint Research Centre (JRC).

Indexed as

DNA sequencinggenetically modified organismsgenetic stabilitymolecular characterisationnext‐generation sequencingNGSrisk assessment

Identifiers

PMID38634010
PMCPMC11022705
OpenAlexW4394893747

What Socratic holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.