Evidence map›Paper›PMID 38645367›Full record

ArticleACS omega2024

Improving Access to Anti-HDV Testing: Development and Validation of an Affordable In-House ELISA Assay.

Iram Amin, Shazia Rafique, Amjad Ali, Nadeem Ahmed, Muhammad Shahid, Samia Afzal, Saad Tahir, Muhammad Waqas, Sadia Bibi, Abdallah M Elgorban and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 3 countries.

Iram AminCentre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore 54590, Pakistan.
Shazia RafiqueCentre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore 54590, Pakistan.ORCID https://orcid.org/0000-0002-0575-8246
Amjad AliDepartment of Biotechnology and Genetic Engineering, Hazara University Mansehra, Mansehra 2100, Pakistan.
Nadeem AhmedCentre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore 54590, Pakistan.
Muhammad ShahidCentre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore 54590, Pakistan.
Samia AfzalCentre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore 54590, Pakistan.
Saad TahirCentre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore 54590, Pakistan.
Muhammad WaqasDepartment of Biotechnology and Genetic Engineering, Hazara University Mansehra, Mansehra 2100, Pakistan.ORCID https://orcid.org/0000-0001-6115-9689
Sadia BibiDepartment of Botany, University of Malakand, Dir (Lower), Chakdara 18800, Khyber Pakhtunkhwa, Pakistan.
Abdallah M ElgorbanDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Muhammad IdreesCentre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore 54590, Pakistan.
Masaud ShahDepartment of Physiology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.
Asad SyedDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
University of the Punjab · PKHazara University · PKKing Saud University · SAAjou University · KRUniversity of Malakand · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In certain low-income nations, the hepatitis Delta virus and hepatitis B virus (HBV) pose a serious medical burden, where the prevalence of hepatitis B surface antigen (HBsAg) is greater than 8%. Especially in rural places, irregular diagnostic exams are the main restriction and reason for underestimation. Utilizing serum samples from a Pakistani isolate, an internal ELISA for the quick identification of anti-HDV was created, and the effectiveness of the test was compared to a commercial diagnostic kit. HDV-positive serum samples were collected, and a highly antigenic domain of HDAg antigen was derived from them. This antigenic HDAg was expressed in a bacterial expression system, purified by Ni-chromatography, and confirmed by SDS-PAGE and Western blot analysis. The purified antigen was utilized to develop an in-house ELISA assay for anti-HDV antibody detection of the patient's serum samples at very low cost. Purified antigens and positive and negative controls can detect anti-HDV (antibodies) in ELISA plates. The in-house developed kit's efficiency was compared with that of a commercial kit (Witech Inc., USA) by the mean optical density values of both kits. No significant difference was observed (a

Identifiers

PMID38645367
PMCPMC11024977
OpenAlexW4393998409

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.