Evidence map›Paper›PMID 38682273›Full record

ArticleDevelopment (Cambridge, England)2024

Optical coherence tomography-guided Brillouin microscopy highlights regional tissue stiffness differences during anterior neural tube closure in the Mthfd1l murine mutant.

Yogeshwari S Ambekar, Carlo Donato Caiaffa, Bogdan J Wlodarczyk, Manmohan Singh, Alexander W Schill, John W Steele, Jitao Zhang, Salavat R Aglyamov, Giuliano Scarcelli, Richard H Finnell and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yogeshwari S AmbekarDepartment of Biomedical Engineering, University of Houston, Houston, TX 77204, USA.ORCID 0000-0003-4266-4924
Carlo Donato CaiaffaCenter for Precision Environmental Health, Baylor College of Medicine, Houston, TX 77030, USA.
Bogdan J WlodarczykCenter for Precision Environmental Health, Baylor College of Medicine, Houston, TX 77030, USA.
Manmohan SinghDepartment of Biomedical Engineering, University of Houston, Houston, TX 77204, USA.
Alexander W SchillDepartment of Biomedical Engineering, University of Houston, Houston, TX 77204, USA.
John W SteeleCenter for Precision Environmental Health, Baylor College of Medicine, Houston, TX 77030, USA.
Jitao ZhangDepartment of Biomedical Engineering, Wayne State University, Detroit, MI 48201, USA.
Salavat R AglyamovDepartment of Mechanical Engineering, University of Houston, Houston, TX 77204, USA.
Giuliano ScarcelliFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Richard H FinnellCenter for Precision Environmental Health, Baylor College of Medicine, Houston, TX 77030, USA.ORCID 0000-0002-5962-8754
Kirill V LarinDepartment of Biomedical Engineering, University of Houston, Houston, TX 77204, USA.ORCID 0000-0002-5532-5027

Funding

Research Programming ModuleP30EY007551 · NEI · UNIVERSITY OF HOUSTON · PI Nimesh Bhikhu Patel · 1988 to 2026
$16.4M
INTERVENTION STRATEGIES FOR NON-FOLATE RESPONSIVE NEURAL TUBE DEFECTSR01HD083809 · NICHD · UNIVERSITY OF TEXAS AT AUSTIN · PI FINNELL, RICHARD H. · 2016 to 2024
$5.1M
Biomechanics of Neural Tube Development using Brillouin-OCT MultimodalityR01HD095520 · NICHD · UNIVERSITY OF HOUSTON · PI FINNELL, RICHARD H., LARIN, KIRILL V · 2018 to 2022
$3.3M
NEI NIH HHS P30 EY007551NICHD NIH HHS R01 HD083809NICHD NIH HHS R01 HD095520NIH HHS HD095520NIH HHS R01 HD095520
6 · The paper itself

Abstract

Neurulation is a highly synchronized biomechanical process leading to the formation of the brain and spinal cord, and its failure leads to neural tube defects (NTDs). Although we are rapidly learning the genetic mechanisms underlying NTDs, the biomechanical aspects are largely unknown. To understand the correlation between NTDs and tissue stiffness during neural tube closure (NTC), we imaged an NTD murine model using optical coherence tomography (OCT), Brillouin microscopy and confocal fluorescence microscopy. Here, we associate structural information from OCT with local stiffness from the Brillouin signal of embryos undergoing neurulation. The stiffness of neuroepithelial tissues in Mthfd1l null embryos was significantly lower than that of wild-type embryos. Additionally, exogenous formate supplementation improved tissue stiffness and gross embryonic morphology in nullizygous and heterozygous embryos. Our results demonstrate the significance of proper tissue stiffness in normal NTC and pave the way for future studies on the mechanobiology of normal and abnormal embryonic development.

Indexed as

Neural TubeNeurulationTomography, Optical CoherenceAnimalsBiomechanical PhenomenaEmbryo, MammalianFemaleFormatesFormate-Tetrahydrofolate LigaseMethylenetetrahydrofolate Dehydrogenase (NADP)MiceMice, KnockoutMicroscopy, ConfocalMutationNeural Tube DefectsFormatesFormate-Tetrahydrofolate Ligaseformic acidMethylenetetrahydrofolate Dehydrogenase (NADP)BiomechanicsBrillouin microscopyMouseNeural tube closureNeural tube defects

Identifiers

PMID38682273
PMCPMC11165724

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.