Evidence map›Paper›PMID 38694445›Full record

ArticleRegenerative therapy2024

A novel cell source for therapy of knee osteoarthritis using atelocollagen microsphere-adhered adipose-derived stem cells: Impact of synovial fluid exposure on cell activity.

Takuya Sakamoto, Atsushi Fuku, Tetsuhiro Horie, Hironori Kitajima, Yuka Nakamura, Ikuhiro Tanida, Hiroshi Sunami, Hiroaki Hirata, Yoshiyuki Tachi, Yasuo Iida and 8 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 4 institutions in 1 country.

Takuya SakamotoMedical Research Institute, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Atsushi FukuDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Tetsuhiro HorieMedical Research Institute, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Hironori KitajimaDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Yuka NakamuraMedical Research Institute, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Ikuhiro TanidaGenome Biotechnology Laboratory, Kanazawa Institute of Technology, Hakusan, 924-0838, Ishikawa, Japan.
Hiroshi SunamiFaculty of Medicine, Advanced Medical Research Center, University of the Ryukyus, 207 Uehara, Nishihara, Nakagami, Okinawa, 903-0215, Japan.
Hiroaki HirataDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Yoshiyuki TachiDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Yasuo IidaDepartment of Mathematics, Division of General Education, Kanazawa Medical University, Kahoku, Ishikawa, 920-0293, Japan.
Sohsuke YamadaCenter for Regenerative Medicine, Kanazawa Medical University Hospital, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Naoki YamamotoSupport Office for Bioresource Research, Center for Translational Research, Translational Research Headquarters, Fujita Health University, Toyoake, 470-1192, Aichi, Japan.
Yusuke ShimizuDepartment of Plastic and Reconstructive Surgery, Graduate School of Medicine, University of the Ryukyus, Nakagami, 903-0215, Okinawa, Japan.
Yasuhito IshigakiMedical Research Institute, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Toru IchisekiMedical Research Institute, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Ayumi KaneujiDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Satoshi OsawaGenome Biotechnology Laboratory, Kanazawa Institute of Technology, Hakusan, 924-0838, Ishikawa, Japan.
Norio KawaharaDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Kanazawa Medical University · JPKanazawa Institute of Technology · JPUniversity of the Ryukyus · JPFujita Health University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Administration of adipose-derived stem cells (ADSCs) into the joint cavity has been shown to alleviate the symptoms of knee osteoarthritis (OA) by releasing exosomes and anti-inflammatory cytokines. However, the therapeutic effect of these cells is limited by their rapid disappearance after administration. Thus, it is necessary to prolong cell survival in the joint cavity. This study aimed to investigate the potential application of ADSCs adhered to atelocollagen microspheres (AMSs) for cell therapy of knee OA. Methods: ADSCs were cultured for 2, 4, and 7 days in AMS suspension or adherent culture dishes. The supernatants were analyzed for IL-10 and exosome secretion via enzyme-linked immunosorbent assay and Nanosight. The effect of AMS was compared with that of adherent-cultured ADSCs (2D-cultured ADSCs) using transcriptome analysis. Moreover, the solubility of AMS and viability of ADSCs were evaluated using synovial fluid (SF) from patients with knee OA. Results: Compared with 2D-cultured ADSCs, AMS-cultured ADSCs exhibited a significant increase in secretion of exosomes and IL-10, and the expression of several genes involved in extracellular matrix and immune regulation were altered. Furthermore, when AMS-cultured ADSCs were cultured in SF from knee OA patients to mimic the intra-articular environment, the SF dissolved the AMSs and released viable ADSCs. In addition, AMS-cultured ADSCs showed significantly higher long-term cell viability than 2D-cultured ADSCs. Conclusion: Increased survival of AMS-adhered ADSCs was observed in the intra-articular environment, and AMSs were found to gradually dissipate. These results suggest that AMS-adhered ADSCs are promising source for cell therapy of knee OA.

Indexed as

Adipose-derived stem cellAtelocollagen microsphereExosomeIL-10Knee osteoarthritisRNA-seq

Identifiers

PMID38694445
PMCPMC11061654
OpenAlexW4395061065

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.