Evidence map›Paper›PMID 38698749›Full record

ArticleCurrent protein & peptide science2024

Dominant Circulating Cell-free Mycobacterial Proteins in In-use Machining Fluid and their Antigenicity Potential.

Harish Chandra, Bethany Ahlers, Ying Wai Lam, Jagjit S Yadav

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Article in Current protein & peptide science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Harish ChandraPulmonary/Microbial Pathogenesis and Immunotoxicology Laboratory, Department of Environmental and Public Health Sciences, University of Cincinnati College of Medicine, Cincinnati, OH45267-0056, USA.ORCID 0000-0002-6088-6152
Bethany AhlersVermont Biomedical Research Network Proteomics Facility, University of Vermont, Burlington, VT05405, USA.
Ying Wai LamVermont Biomedical Research Network Proteomics Facility, University of Vermont, Burlington, VT05405, USA.
Jagjit S YadavPulmonary/Microbial Pathogenesis and Immunotoxicology Laboratory, Department of Environmental and Public Health Sciences, University of Cincinnati College of Medicine, Cincinnati, OH45267-0056, USA.ORCID 0000-0001-5036-3609

Funding

Circulating Cell-free Antigens for Monitoring of Machining FluidsR21OH011826 · OH · UNIVERSITY OF CINCINNATI · PI YADAV, JAGJIT S · 2020 to 2021
$441k
CDC HHSNIOSH CDC HHS 1R21OH011826NIOSH CDC HHS R21 OH011826University of Cincinnati T42/OH008432-10Vermont Agricultural Experiment Station RRID:SCR_018667
6 · The paper itself

Abstract

backgroundOccupational exposure to industrial Metalworking Fluid (MWF) colonized by

objectiveTo detect and identify dominant MI proteins and antigens directly from the field-drawn in-use MWF using an integrated immunoproteomic-immunoinformatic approach.

methodsAn MI-positive MWF selected by DNA-based screening of several field-drawn MWF samples was cultured to isolate the colonizing strain and profiled for dominant circulating cell-free (ccf) MI proteins, including antigens using an integrated immunoproteomic (1D- and 2Dgel fractionation of seroreactive proteins combined with shotgun proteomic analysis using LC-MS/MS) and immunoinformatic strategy.

resultsA new MI strain (MJY-27) was identified. The gel fractionated MI protein bands (1Dgel) or spots (2D-gel) seroreactive with anti-MI sera probes (Rabbit and Patient sera) yielded 86 MI proteins, 29 of which showed peptide abundance. T-cell epitope analysis revealed high (90-100%) binding frequency for HLA-I & II alleles for 13 of the 29 proteins. Their antigenicity analysis revealed the presence of 6 to 37 antigenic determinants. Interestingly, one of the identified candidates corresponded to an experimentally validated strong B- and T-cell antigen (AgD) from our laboratory culture-based studies.

conclusionThis first report on dominant proteins, including putative antigens of M. immunogenum prevalent in field in-use MWF, is a significant step towards the overall goal of developing fluid monitoring for exposure and disease risk assessment for HP development in machining environments.

Indexed as

Antigens, BacterialBacterial ProteinsAlveolitis, Extrinsic AllergicEpitopes, T-LymphocyteHumansMetallurgyMycobacteriumOccupational ExposureProteomicsTandem Mass SpectrometryAntigens, BacterialBacterial ProteinsEpitopes, T-LymphocyteepitopeHLA allele.hypersensitivity pneumonitisMetalworking fluidMycobacterium immunogenumproteinT-cell antigen

Identifiers

PMID38698749

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.