Evidence map›Paper›PMID 38703129›Full record

ArticleJournal of veterinary internal medicine

Salivary metabolomic identification of biomarker candidates for oral melanoma and oral squamous cell carcinoma in dogs.

Sekkarin Ploypetch, Xian Luo, Shuang Zhao, Sittiruk Roytrakul, Liang Li, Gunnaporn Suriyaphol

Abstract read
In one paragraph

Article in Journal of veterinary internal medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sekkarin PloypetchDepartment of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand.ORCID https://orcid.org/0000-0003-4985-2565
Xian LuoThe Metabolomics Innovation Centre, University of Alberta, Edmonton, Alberta, Canada.ORCID https://orcid.org/0000-0003-1026-845X
Shuang ZhaoThe Metabolomics Innovation Centre, University of Alberta, Edmonton, Alberta, Canada.ORCID https://orcid.org/0000-0002-1943-8542
Sittiruk RoytrakulFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand.ORCID https://orcid.org/0000-0003-3696-8390
Liang LiThe Metabolomics Innovation Centre, University of Alberta, Edmonton, Alberta, Canada.ORCID https://orcid.org/0000-0002-9347-2108
Gunnaporn SuriyapholBiochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.ORCID https://orcid.org/0000-0002-8961-5970

Funding

An Overseas Research Experience Scholarship for Graduate StudentCanada Foundation for InnovationGenome CanadaThe 100th Anniversary of Chulalongkorn University for a Doctoral ScholarshipThe 90th Anniversary of Chulalongkorn University ScholarshipThe Natural Sciences and Engineering Research Council of CanadaTRF Research Career Development Grant RSA5980053
6 · The paper itself

Abstract

backgroundOral melanoma (OM) and oral squamous cell carcinoma (OSCC) are frequently diagnosed in dogs, presenting a challenge in distinguishing them from benign oral tumors (BN). Salivary metabolomic biomarkers offer a practical solution because of saliva's direct contact with tumors and the noninvasive nature of collection.

objectiveAssess the diversity and abundance of the salivary metabolome in dogs with BN, OM, and OSCC using amine/phenol submetabolome analysis and high-performance chemical isotope labeling liquid chromatography-mass spectrometry (CIL LC-MS). ANIMALS: Study included 11 BN, 24 OM, 10 OSCC, and 20 healthy control dogs.

methodsCase-control cross-sectional study was conducted to assess salivary submetabolic profiles in dogs with BN, OM, and OSCC and healthy dogs. Samples were labeled with

resultsDistinct clusters and significant differences in metabolite concentrations were observed among the oral cancer, BN, and control groups. A total of 154 and 66 metabolites showed significantly altered concentrations, particularly in OM and OSCC, respectively, when compared with BN (Padj < .05). Potential metabolic biomarkers were identified for each cancer, including decreased concentrations of seryl-arginine and sarcosine in OSCC. Moreover, high-confidence putative metabolites were identified, including an increase in tryptophyl-threonine and a decrease in 1,2-dihydroxynapthalene-6-sulfonic acid and hydroxyprolyl-hydroxyproline for OM. CONCLUSIONS AND CLINICAL IMPORTANCE: We identified high coverage of the amine/phenol submetabolome, including seryl-arginine, and sarcosine, in OSCC. Our findings emphasize the potential of these biomarkers for distinguishing between oral OSCC and BN in dogs.

Indexed as

Biomarkers, TumorCarcinoma, Squamous CellDog DiseasesMelanomaMouth NeoplasmsSalivaAnimalsCase-Control StudiesChromatography, LiquidCross-Sectional StudiesDogsFemaleMaleMetabolomeMetabolomicsBiomarkers, Tumorcanine oral melanomacanine oral squamous cell carcinomametabolomic biomarkerssaliva

Identifiers

PMID38703129
PMCPMC11256132

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.