Evidence map›Paper›PMID 38711079›Full record

ArticleBMC musculoskeletal disorders2024

YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs.

Tianyi Yuan, Hongjiang Liu, Maimaitiyibubaji Abudoukadier, Zengqiang Yang, Zhiheng Zhou, Yong Cui

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Article in BMC musculoskeletal disorders, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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3 · Its place in the literature

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3 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Tianyi YuanThe Fifth Clinical Medical College of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830011, China.
Hongjiang LiuThe Fifth Clinical Medical College of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830011, China.
Maimaitiyibubaji AbudoukadierThe Fifth Clinical Medical College of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830011, China.
Zengqiang YangThe Fifth Clinical Medical College of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830011, China.
Zhiheng ZhouThe Fifth Clinical Medical College of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830011, China.
Yong CuiDepartment of Orthopedic Center, The Fifth Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi, 830011, China. 1511021831@qq.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundWith the increasing incidence of steroid-induced necrosis of the femoral head (SNFH), numerous scholars have investigated its pathogenesis. Current evidence suggests that the imbalance between lipogenesis and osteoblast differentiation in bone marrow mesenchymal stem cells (BMSCs) is a key pathological feature of SNFH. MicroRNAs (miRNAs) have strong gene regulatory effects and can influence the direction of cell differentiation. N6-methyladenosine (m6A) is a prevalent epigenetic modification involved in diverse pathophysiological processes. However, knowledge of how miRNAs regulate m6A-related factors that affect BMSC differentiation is limited.

objectiveWe aimed to investigate the role of miR27a in regulating the expression of YTHDF2 in BMSCs.

methodsWe compared miR27a, YTHDF2, and total m6A mRNA levels in SNFH-affected and control BMSCs. CCK-8 and TUNEL assays were used to assess BMSC proliferation and apoptosis. Western blotting and qRT‒PCR were used to measure the expression of osteogenic (ALP, RUNX2, and OCN) and lipogenic (PPARγ and C/EBPα) markers. Alizarin Red and Oil Red O staining were used to quantify osteogenic and lipogenic differentiation, respectively. miR27a was knocked down or overexpressed to evaluate its impact on BMSC differentiation and its relationship with YTHDF2. Bioinformatics analyses identified YTHDF2 as a differentially expressed gene in SNFH (ROC analysis) and revealed potential signaling pathways through GSEA. The effects of YTHDF2 silencing on the lipogenic and osteogenic functions of BMSCs were assessed.

resultsmiR27a downregulation and YTHDF2 upregulation were observed in the SNFH BMSCs. miR27a knockdown/overexpression modulated YTHDF2 expression, impacting BMSC differentiation. miR27a silencing decreased m6A methylation and promoted osteogenic differentiation, while YTHDF2 silencing exerted similar effects. GSEA suggested potential signaling pathways associated with YTHDF2 in SNFH.

conclusionmiR27a regulates BMSC differentiation through YTHDF2, affecting m6A methylation and promoting osteogenesis. This finding suggests a potential therapeutic target for SNFH.

Indexed as

Cell DifferentiationMesenchymal Stem CellsMicroRNAsOsteogenesisRNA-Binding ProteinsAdenosineAnimalsApoptosisCell ProliferationCells, CulturedFemur Head NecrosisHumansLipogenesisMaleMethylationAdenosineMicroRNAsN-methyladenosineRNA-Binding ProteinsYTHDF2 protein, humanBone marrow mesenchymal stem cellsmiRNAN6-methyladenosineSteroid-induced necrosis of the femoral head

Identifiers

PMID38711079
PMCPMC11071322

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.