Evidence map›Paper›PMID 38715944›Full record

ArticleiScience2024

Rab27a promotes degradation of West Nile virus E protein in the lysosome.

Shintaro Kobayashi, Seira Kawai, Yukine Fukuda, Haruto Eguchi, Keisuke Maezono, Passawat Thammahakin, Hirofumi Sawa, Hiroaki Kariwa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shintaro KobayashiLaboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Seira KawaiLaboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Yukine FukudaLaboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Haruto EguchiLaboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Keisuke MaezonoLaboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Passawat ThammahakinLaboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Hirofumi SawaHokkaido University, Institute for Vaccine Research and Development (HU-IVReD), Sapporo, Hokkaido 001-0021, Japan.
Hiroaki KariwaLaboratory of Public Health, Faculty of Veterinary medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rab27a, a Rab family small GTPases, plays an important role in the trafficking and secretion of the intracellular proteins and has been reported to promote various viral multiplication. However, whether Rab27a is involved in West Nile virus (WNV) multiplication is unknown. This study examined the ability of Rab27a to suppress WNV multiplication. The inhibition of Rab27a expression increased viral multiplication and the intracellular levels of WNV structural proteins, E and prM proteins. Rab27a partially colocalized with E protein, mainly in the perinuclear region, while inhibition of Rab27a expression resulted in diffuse subcellular localization of E protein. In addition, some of the perinuclear E protein colocalized with the lysosomal marker LAMP1, and inhibition of lysosomal acidification increased intracellular levels of Rab27a and E proteins. These observations suggested that Rab27a inhibits WNV multiplication by inducing the degradation of viral protein in lysosomes.

Indexed as

Cell biologyVirology

Identifiers

PMID38715944
PMCPMC11075059

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.