Evidence map›Paper›PMID 38748756›Full record

ArticlePloS one2024

Vitamin D regulates COVID-19 associated severity by suppressing the NLRP3 inflammasome pathway.

Bariaa Khalil, Narjes Saheb Sharif-Askari, Shirin Hafezi, Fatemeh Saheb Sharif-Askari, Fatme Al Anouti, Qutayba Hamid, Rabih Halwani

Abstract read
In one paragraph

Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Bariaa KhalilResearch Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.
Narjes Saheb Sharif-AskariResearch Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.
Shirin HafeziResearch Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.
Fatemeh Saheb Sharif-AskariResearch Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.
Fatme Al AnoutiCollege of Natural and Health Sciences, Zayed University, Abu Dhabi, United Arab Emirates.
Qutayba HamidResearch Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.
Rabih HalwaniResearch Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.ORCID https://orcid.org/0000-0002-6516-7771

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe role of vitamin D3 (VitD3) in modulating innate and adaptive immunity has been reported in different disease contexts. Since the start of the coronavirus disease-2019 (COVID-19) pandemic, the role of VitD3 has been highlighted in many correlational and observational studies. However, the exact mechanisms of action are not well identified. One of the mechanisms via which VitD3 modulates innate immunity is by regulating the NLRP3-inflammasome pathway, being a main underlying cause of SARS-CoV-2-induced hyperinflammation. AIMS AND MAIN

methodsBlood specimens of severe COVID-19 patients with or without VitD3 treatment were collected during their stay in the intensive care unit and patients were followed up for 29 days. qPCR, western blot, and ELISA were done to investigate the mechanism of action of VitD3 on the NLRP3 inflammasome activation. KEY

findingsWe here report the ability of VitD3 to downregulate the NLRP3-inflammsome pathway in severe COVID-19 patients. Lower inflammasome pathway activation was observed with significantly lower gene and protein expression of NLRP3, cleaved caspase-1, ASC and IL-1β among severe COVID-19 patients treated with VitD3. The reduction of the inflammasome pathway was associated with a reduction in disease severity markers and enhancement of type I IFN pathway. SIGNIFICANCE: Our data reveals an important anti-inflammatory effect of VitD3 during SARS-CoV-2 infection. Further investigations are warranted to better characterize the ability of VitD3 to control disease pathogenesis and prevent progression to severe states. This will allow for a more efficient use of a low cost and accessible treatment like VitD3.

Indexed as

CholecalciferolCOVID-19COVID-19 Drug TreatmentInflammasomesNLR Family, Pyrin Domain-Containing 3 ProteinSARS-CoV-2FemaleHumansInterleukin-1betaMalePatient AcuitySeverity of Illness IndexSignal TransductionVitamin DCholecalciferolInflammasomesInterleukin-1betaNLR Family, Pyrin Domain-Containing 3 ProteinVitamin D

Identifiers

PMID38748756
PMCPMC11095707

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.