Evidence map›Paper›PMID 38758323›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Measuring Laccase Activity and Melanin Production in Cryptococcus neoformans.

Patrícia Albuquerque, Herdson Renney de Sousa, Stefânia de Oliveira Frazão, Larissa Verginia do Nascimento Miranda, Hugo Costa Paes, Ildinete Silva Pereira, André Moraes Nicola

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Patrícia AlbuquerqueFaculty of Ceilândia, University of Brasília, Brasília, DF, Brazil. palbuquerque@unb.br.
Herdson Renney de SousaFaculty of Medicine, University of Brasília, Brasília, DF, Brazil.
Stefânia de Oliveira FrazãoInstitute of Biology, Department of Cell Biology, University of Brasília, Brasília, DF, Brazil.
Larissa Verginia do Nascimento MirandaInstitute of Biology, Department of Cell Biology, University of Brasília, Brasília, DF, Brazil.
Hugo Costa PaesFaculty of Medicine, University of Brasília, Brasília, DF, Brazil.
Ildinete Silva PereiraInstitute of Biology, Department of Cell Biology, University of Brasília, Brasília, DF, Brazil.
André Moraes NicolaFaculty of Medicine, University of Brasília, Brasília, DF, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Melanin is a complex dark pigment synthetized by the phenoloxidase enzyme laccase in Cryptococcus neoformans. In vitro, this enzyme oxidizes exogenous catecholamines to produce melanin that may be secreted or incorporated into the fungal cell wall. This pigment has multiple roles in C. neoformans virulence during its interaction with different hosts and probably also in protecting fungal cells in the environment against predation and oxidative and radiation stresses, among others. However, it is important to note that laccase also has melanin-independent roles in C. neoformans interactions with host cells. In this chapter, we describe a quantitative laccase assay and a method for evaluating the kinetics of melanin production in C. neoformans colonies.

Indexed as

Cryptococcus neoformansLaccaseMelaninsEnzyme AssaysLaccaseMelaninsCryptococcosisCryptococcus neoformansDensitometryMelaninMelanizationPhotography

Identifiers

PMID38758323

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.