Evidence map›Paper›PMID 38766072›Full record

ArticlebioRxiv : the preprint server for biology2024

Accelerated protein retention expansion microscopy using microwave radiation.

Meghan R Bullard, Juan Carlos Martinez Cervantes, Norisha B Quaicoe, Amanda Jin, Danya A Adams, Jessica M Lin, Elena Iliadis, Tess M Seidler, Isaac Cervantes-Sandoval, Hai-Yan He

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Meghan R BullardDepartment of Biology, Georgetown University, Washington, DC 20057.
Juan Carlos Martinez CervantesDepartment of Biology, Georgetown University, Washington, DC 20057.
Norisha B QuaicoeDepartment of Biology, Georgetown University, Washington, DC 20057.
Amanda JinDepartment of Biology, Georgetown University, Washington, DC 20057.
Danya A AdamsDepartment of Biology, Georgetown University, Washington, DC 20057.
Jessica M LinDepartment of Biology, Georgetown University, Washington, DC 20057.
Elena IliadisDepartment of Biology, Georgetown University, Washington, DC 20057.
Tess M SeidlerDepartment of Biology, Georgetown University, Washington, DC 20057.
Isaac Cervantes-SandovalDepartment of Biology, Georgetown University, Washington, DC 20057.
Hai-Yan HeDepartment of Biology, Georgetown University, Washington, DC 20057.ORCID 0000-0002-6692-6012

Funding

Institutional Career Development CoreKL2TR001432 · NCATS · GEORGETOWN UNIVERSITY · PI UMANS, JASON G · 2015 to 2024
$7.5M
Nascent protein degradation-based fast homeostatic mechanism mediated by neuronal membrane proteasomesR01NS133441 · NINDS · GEORGETOWN UNIVERSITY · PI Haiyan He · 2023 to 2026
$1.5M
NCATS NIH HHS KL2 TR001432NINDS NIH HHS R01 NS133441
6 · The paper itself

Abstract

Protein retention expansion microscopy (ExM) retains genetically encoded fluorescent proteins or antibody-conjugated fluorescent probes in fixed tissue and isotropically expands the tissue through a swellable polymer network to allow nanoscale (<70 nm) resolution on diffraction-limited confocal microscopes. Despite numerous advantages ExM brings to biological studies, the full protocol is time-consuming and can take multiple days to complete. Here, we adapted the ExM protocol to the vibratome-sectioned brain tissue of

Indexed as

Drosophila melanogasterExpansion microscopyimmunohistochemistrymicrowaveneural tissueXenopus laevis tadpoles

Identifiers

PMID38766072
PMCPMC11100821

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.