Evidence map›Paper›PMID 38787479›Full record

ArticleMolecular biology reports2024

Silencing of the T-type voltage-gated calcium channel α

Jiahao Yang, Siyu Zhou, Ziqi Yang, Xuqi Shi, Haoran Liu, Zhuhong Yang, Deliang Peng, Zhong Ding, Shan Ye

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Jiahao YangCollege of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Siyu ZhouCollege of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Ziqi YangCollege of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Xuqi ShiCollege of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Haoran LiuCollege of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Zhuhong YangCollege of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Deliang PengState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Zhong DingCollege of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China. dingzh@hunau.net.
Shan YeCollege of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China. yeshan@hunau.edu.cn.

Funding

National Key Research and Development Program of China No.2023YFD1400013National Natural Science Foundation of China No. 31872038Natural Science Foundation of Hunan Province No.2022JJ30302
6 · The paper itself

Abstract

backgroundT-type calcium channels, characterized as low-voltage activated (LVA) calcium channels, play crucial physiological roles across a wide range of tissues, including both the neuronal and nonneuronal systems. Using in situ hybridization and RNA interference (RNAi) techniques in vitro, we previously identified the tissue distribution and physiological function of the T-type calcium channel α METHODS AND

resultsTo further characterize the functional role of DdCα1G, we employed a combination of immunohistochemistry and fungus-mediated RNAi and found that DdCα1G was clearly distributed in stylet-related tissue, oesophageal gland-related tissue, secretory-excretory duct-related tissue and male spicule-related tissue. Silencing DdCα1G led to impairments in the locomotion, feeding, reproductive ability and protein secretion of nematodes. To confirm the defects in behavior, we used phalloidin staining to examine muscle changes in DdCα1G-RNAi nematodes. Our observations demonstrated that defective behaviors are associated with related muscular atrophy.

conclusionOur findings provide a deeper understanding of the physiological functions of T-type calcium channels in plant-parasitic nematodes. The T-type calcium channel can be considered a promising target for sustainable nematode management practices.

Indexed as

ActinsCalcium Channels, T-TypeRNA InterferenceAnimalsFungiGene SilencingMaleActinsCalcium Channels, T-TypeDitylenchus destructorF-actinPhysiological functionPlant parasitic nematodesT-type voltage-gated calcium channels (VGCCs)

Identifiers

PMID38787479

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.