Evidence map›Paper›PMID 38798409›Full record

ArticlebioRxiv : the preprint server for biology2024

Effects of alcohol consumption and tobacco smoking on the composition of the ensemble of drug metabolizing enzymes and transporters in human liver.

Kari A Gaither, Dilip Kumar Singh, Guihua Yue, Julia Trudeau, Kannapiran Ponraj, Nadezhda Y Davydova, Philip Lazarus, Dmitri R Davydov, Bhagwat Prasad

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Kari A GaitherDepartment of Pharmaceutical Sciences, Washington State University, Spokane, WA, 99202.ORCID 0000-0003-2014-4362
Dilip Kumar SinghDepartment of Pharmaceutical Sciences, Washington State University, Spokane, WA, 99202.ORCID 0000-0001-7248-6714
Guihua YueDepartment of Pharmaceutical Sciences, Washington State University, Spokane, WA, 99202.
Julia TrudeauDepartment of Pharmaceutical Sciences, Washington State University, Spokane, WA, 99202.
Kannapiran PonrajDepartment of Chemistry, Washington State University, Pullman, WA, 99164.ORCID 0000-0002-7195-5432
Nadezhda Y DavydovaDepartment of Chemistry, Washington State University, Pullman, WA, 99164.
Philip LazarusDepartment of Pharmaceutical Sciences, Washington State University, Spokane, WA, 99202.
Dmitri R DavydovDepartment of Chemistry, Washington State University, Pullman, WA, 99164.ORCID 0000-0001-8520-0033
Bhagwat PrasadDepartment of Pharmaceutical Sciences, Washington State University, Spokane, WA, 99202.ORCID 0000-0002-9090-0912

Funding

Inter-Enzyme Crosstalk in the Cytochrome P450 Ensemble: Implications for the Effects of Alcohol on Drug Metabolism and Alcohol-Drug InteractionsR01AA030155 · NIAAA · WASHINGTON STATE UNIVERSITY · PI DAVYDOV, DMITRI R, PRASAD, BHAGWAT · 2022 to 2025
$2.0M
NIAAA NIH HHS R01 AA030155
6 · The paper itself

Abstract

We examined the effect of alcohol consumption and smoking on the abundance of drug-metabolizing enzymes and transporters (DMET) in human liver microsomes (HLM) isolated from liver tissues of 94 donors. Global proteomics analysis was performed and DMET protein levels were analyzed in relation to alcohol consumption levels, smoking history, and sex using non-parametric tests (p-value ≤ 0.05; cutoff of 1.25-fold change, FC). The examination of the alcohol-induced changes was further enforced by correlational analysis, where we used arbitrary alcohol consumption grade (ACG) scaling from 0 to 4 to establish a set of protein markers. We elaborated a provisional index of alcohol exposure (PIAE) based on a combination of relative abundances of four proteins (ER chaperone HSPA5, protein disulfide isomerases PDIA3 and P4HB, and cocaine esterase CES2) best correlating with ACG. The PIAE index was then used to find its correlations with the abundances of DMET proteins. Our results demonstrate considerable alcohol-induced changes in composition of the pool of cytochrome P450 enzymes in HLM. We observed significantly increased abundances of CYP2E1, CYP2B6, CYP2J2, and NADPH-cytochrome P450 reductase. In contrast, CYP1A2, CYP2C8, CYP2C9, CYP4A11, and cytochrome b

Indexed as

alcohol exposurecytochromes P450drug metabolismhuman liver microsomesliver proteometobacco smoking

Identifiers

PMID38798409
PMCPMC11118358

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.