Evidence map›Paper›PMID 38802708›Full record

ArticleWorld journal of microbiology & biotechnology2024

Improvement of thermostability by increasing rigidity in the finger regions and flexibility in the catalytic pocket area of Pseudoalteromonas porphyrae κ-carrageenase.

Zeping Du, Xiaoyi Huang, Hebin Li, Mingjing Zheng, Tao Hong, Zhipeng Li, Xiping Du, Zedong Jiang, Hui Ni, Qingbiao Li and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in World journal of microbiology & biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zeping DuCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Xiaoyi HuangCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Hebin LiDepartment of Pharmacy, Xiamen Medical College, Xiamen, 361008, China.
Mingjing ZhengCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Tao HongCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Zhipeng LiCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Xiping DuCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Zedong JiangCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Hui NiCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Qingbiao LiCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Yanbing ZhuCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China. yanbingzhu@jmu.edu.cn.

Funding

National Natural Science Foundation of China 22178142National Natural Science Foundation of China 41976124Natural Science Foundation of Fujian Province 2023J011652
6 · The paper itself

Abstract

Poor thermostability reduces the industrial application value of κ-carrageenase. In this study, the PoPMuSiC algorithm combined with site-directed mutagenesis was applied to improve the thermostability of the alkaline κ-carrageenase from Pseudoalteromonas porphyrae. The mutant E154A with improved thermal stability was successfully obtained using this strategy after screening seven rationally designed mutants. Compared with the wild-type κ-carrageenase (WT), E154A improved the activity by 29.4% and the residual activity by 51.6% after treatment at 50 °C for 30 min. The melting temperature (T

Indexed as

Catalytic DomainEnzyme StabilityGlycoside HydrolasesMolecular Dynamics SimulationMutagenesis, Site-DirectedPseudoalteromonasBacterial ProteinsCarrageenanCircular DichroismKineticsProtein ConformationTemperatureBacterial ProteinsCarrageenanGlycoside HydrolasesFinger regionRational designThermostabilityκcarrageenase

Identifiers

PMID38802708

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.