Evidence map›Paper›PMID 38827406›Full record

ArticleiScience2024

Compartmentalization proteomics revealed endolysosomal protein network changes in a goat model of atrial fibrillation.

Thamali Ayagama, Philip D Charles, Samuel J Bose, Barry Boland, David A Priestman, Daniel Aston, Georgina Berridge, Roman Fischer, Adam P Cribbs, Qianqian Song and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Chaperonin in health and disease.Molecular biomedicine · 2026
    Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Thamali AyagamaDepartment of Pharmacology, University of Oxford, Oxford, UK.
Philip D CharlesTarget Discovery Institute, University of Oxford, Oxford, UK.
Samuel J BoseDepartment of Pharmacology, University of Oxford, Oxford, UK.
Barry BolandDepartment of Pharmacology and Therapeutics, University College Cork, Cork, Ireland.
David A PriestmanDepartment of Pharmacology, University of Oxford, Oxford, UK.
Daniel AstonDepartment of Anaesthesia and Critical Care, Royal Papworth Hospital NHS Foundation Trust, Papworth Road, Cambridge CB2 0AY, UK.
Georgina BerridgeTarget Discovery Institute, University of Oxford, Oxford, UK.
Roman FischerTarget Discovery Institute, University of Oxford, Oxford, UK.
Adam P CribbsNuffield Department of Orthopaedics Rheumatology and Musculoskeletal Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Botnar Research Centre, University of Oxford, Headington OX3 7LD, UK.
Qianqian SongDepartment of Pharmacology, University of Oxford, Oxford, UK.
Gary R MiramsCentre for Mathematical Medicine & Biology, Mathematical Sciences, University of Nottingham, Nottingham NG7 2RD, UK.
Kwabena AmponsahCentre for Mathematical Medicine & Biology, Mathematical Sciences, University of Nottingham, Nottingham NG7 2RD, UK.
Lisa HeatherDepartment of Physiology, Anatomy and Genetics, , University of Oxford, South Park Road, Oxford OX1 3PT, UK.
Antony GalioneDepartment of Pharmacology, University of Oxford, Oxford, UK.
Neil HerringDepartment of Physiology, Anatomy and Genetics, , University of Oxford, South Park Road, Oxford OX1 3PT, UK.
Holger KramerMass spectrometry Facility, The MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
Rebecca A CapelDepartment of Pharmacology, University of Oxford, Oxford, UK.
Frances M PlattDepartment of Pharmacology, University of Oxford, Oxford, UK.
Ulrich SchottenDepartments of Physiology and Cardiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
Sander VerheuleDepartments of Physiology and Cardiology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
Rebecca A B BurtonDepartment of Pharmacology, University of Oxford, Oxford, UK.

Funding

British Heart Foundation FS/SCRF/20/32005Medical Research Council MR/V010182/1Wellcome Trust
6 · The paper itself

Abstract

Endolysosomes (EL) are known for their role in regulating both intracellular trafficking and proteostasis. EL facilitate the elimination of damaged membranes, protein aggregates, membranous organelles and play an important role in calcium signaling. The specific role of EL in cardiac atrial fibrillation (AF) is not well understood. We isolated atrial EL organelles from AF goat biopsies and conducted a comprehensive integrated omics analysis to study the EL-specific proteins and pathways. We also performed electron tomography, protein and enzyme assays on these biopsies. Our results revealed the upregulation of the AMPK pathway and the expression of EL-specific proteins that were not found in whole tissue lysates, including GAA, DYNLRB1, CLTB, SIRT3, CCT2, and muscle-specific HSPB2. We also observed structural anomalies, such as autophagic-vacuole formation, irregularly shaped mitochondria, and glycogen deposition. Our results provide molecular information suggesting EL play a role in AF disease process over extended time frames.

Indexed as

Cell biologyMolecular biologyProteomicsTranscriptomics

Identifiers

PMID38827406
PMCPMC11141153

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.