Evidence mapPaperPMID 38837640Full record

ArticleAnnals of clinical and translational neurology2024

SCAR32: Functional characterization and expansion of the clinical-genetic spectrum.

Valentina Naef, Maria Lieto, Sara Satolli, Rosa De Micco, Martina Troisi, Rosa Pasquariello, Stefano Doccini, Flavia Privitera, Alessandro Filla, Alessandro Tessitore and 1 more

Abstract readCase Reports
In one paragraph

Article in Annals of clinical and translational neurology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Neurodevelopmental disorders in childhood-onset hereditary spastic paraplegia type 7: a case series and review of literature.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2026
    Pooled it
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Valentina NaefDepartment Neurobiology and Molecular Medicine, IRCCS Fondazione Stella Maris, Pisa, 56128, Italy.
Maria LietoDepartment of Neurology and Stroke Unit, Ospedale del Mare Hospital, Naples, Italy.
Sara SatolliDepartment Neurobiology and Molecular Medicine, IRCCS Fondazione Stella Maris, Pisa, 56128, Italy.
Rosa De MiccoDepartment of Advanced Medical and Surgical Sciences, University of Campania "Luigi Vanvitelli", Naples, Italy.
Martina TroisiDepartment Neurobiology and Molecular Medicine, IRCCS Fondazione Stella Maris, Pisa, 56128, Italy.
Rosa PasquarielloDepartment Neurobiology and Molecular Medicine, IRCCS Fondazione Stella Maris, Pisa, 56128, Italy.
Stefano DocciniDepartment Neurobiology and Molecular Medicine, IRCCS Fondazione Stella Maris, Pisa, 56128, Italy.
Flavia PriviteraDepartment Neurobiology and Molecular Medicine, IRCCS Fondazione Stella Maris, Pisa, 56128, Italy.
Alessandro FillaDepartment of Neurosciences, Reproductive and Odontostomatological Sciences, Federico II University, Naples, Italy.
Alessandro TessitoreDepartment of Advanced Medical and Surgical Sciences, University of Campania "Luigi Vanvitelli", Naples, Italy.
Filippo Maria SantorelliDepartment Neurobiology and Molecular Medicine, IRCCS Fondazione Stella Maris, Pisa, 56128, Italy.ORCID 0000-0002-1359-9062

Funding

Italian Ministry of Health RF-2019-12370417
6 · The paper itself

Abstract

objectiveBiallelic mutations in PRDX3 have been linked to autosomal recessive spinocerebellar ataxia type 32. In this study, which aims to contribute to the growing body of knowledge on this rare disease, we identified two unrelated patients with mutations in PRDX3. We explored the impact of PRDX3 mutation in patient skin fibroblasts and the role of the gene in neurodevelopment.

methodsWe performed trio exome sequencing that identified mutations in PRDX3 in two unrelated patients. We also performed functional studies in patient skin fibroblasts and generated a "crispant" zebrafish (Danio rerio) model to investigate the role of the gene during nervous system development.

resultsOur study reports two additional patients. Patient 1 is a 19-year-old male who showed a novel homozygous c.525_535delGTTAGAAGGTT (p. Leu176TrpfsTer11) mutation as the genetic cause of cerebellar ataxia. Patient 2 is a 20-year-old male who was found to present the known c.425C>G/p. Ala142Gly variant in compound heterozygosity with the p. Leu176TrpfsTer11 one. While the fibroblast model failed to recapitulate the pathological features associated with PRDX3 loss of function, our functional characterization of the prdx3 zebrafish model revealed motor defects, increased susceptibility to reactive oxygen species-triggered apoptosis, and an impaired oxygen consumption rate.

conclusionsWe identified a new variant, thereby expanding the genetic spectrum of PRDX3-related disease. We developed a novel zebrafish model to investigate the consequences of prdx3 depletion on neurodevelopment and thus offered a potential new tool for identifying new treatment opportunities.

Indexed as

ZebrafishAnimalsFibroblastsHumansMaleMutationSpinocerebellar AtaxiasYoung Adult

Identifiers

PMID38837640
PMCPMC11251466

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.