Evidence mapPaperPMID 38842166Full record

ArticleeLife2024

Endothelial cell signature in muscle stem cells validated by VEGFA-FLT1-AKT1 axis promoting survival of muscle stem cell.

Mayank Verma, Yoko Asakura, Xuerui Wang, Kasey Zhou, Mahmut Ünverdi, Allison P Kann, Robert S Krauss, Atsushi Asakura

Abstract read
In one paragraph

Article in eLife, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Review
  2. Article
  3. Angiogenic Doping: Plausible Yet Difficult to Detect.Sports medicine (Auckland, N.Z.) · 2026
    Review
  4. Review
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  13. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mayank VermaDepartment of Pediatrics & Neurology, Division of Pediatric Neurology, The University of Texas Southwestern Medical Center, Dallas, United States.ORCID https://orcid.org/0000-0003-0167-0842
Yoko AsakuraStem Cell Institute, University of Minnesota Medical School, Minneapolis, United States.ORCID https://orcid.org/0000-0003-4107-4236
Xuerui WangStem Cell Institute, University of Minnesota Medical School, Minneapolis, United States.
Kasey ZhouStem Cell Institute, University of Minnesota Medical School, Minneapolis, United States.
Mahmut ÜnverdiStem Cell Institute, University of Minnesota Medical School, Minneapolis, United States.
Allison P KannDepartment of Cell, Developmental, and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States.ORCID https://orcid.org/0000-0003-0111-9081
Robert S KraussDepartment of Cell, Developmental, and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, United States.ORCID https://orcid.org/0000-0002-7661-3335
Atsushi AsakuraStem Cell Institute, University of Minnesota Medical School, Minneapolis, United States.ORCID https://orcid.org/0000-0001-8078-1027

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM008244 · UNIVERSITY OF MINNESOTA TWIN CITIES · 1988 to 2005
$1.9M
Cadherin-Dependent Regulation of Satellite Cell FunctionR01AR070231 · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · 2025 to 2025
$536k
Muscular Dystrophy Association MDA241600New York State Stem Cell Science NYSTEM-C32561GGNIAMS NIH HHS AR070231NIAMS NIH HHS F30 AR066454NIAMS NIH HHS NIHR01AR062142NIAMS NIH HHS NIHR21AR070319NIAMS NIH HHS R01 AR062142NIAMS NIH HHS R01 AR070231NIAMS NIH HHS R21 AR070319NIGMS NIH HHS T32 GM008244NIH HHS NIHF30AR066454NIH HHS NIHT32-GM008244Regenerative Medicine Minnesota RMM 092319 TR 010
6 · The paper itself

Abstract

Endothelial and skeletal muscle lineages arise from common embryonic progenitors. Despite their shared developmental origin, adult endothelial cells (ECs) and muscle stem cells (MuSCs; satellite cells) have been thought to possess distinct gene signatures and signaling pathways. Here, we shift this paradigm by uncovering how adult MuSC behavior is affected by the expression of a subset of EC transcripts. We used several computational analyses including single-cell RNA-seq (scRNA-seq) to show that MuSCs express low levels of canonical EC markers in mice. We demonstrate that MuSC survival is regulated by one such prototypic endothelial signaling pathway (VEGFA-FLT1). Using pharmacological and genetic gain- and loss-of-function studies, we identify the FLT1-AKT1 axis as the key effector underlying VEGFA-mediated regulation of MuSC survival. All together, our data support that the VEGFA-FLT1-AKT1 pathway promotes MuSC survival during muscle regeneration, and highlights how the minor expression of select transcripts is sufficient for affecting cell behavior.

Indexed as

Cell SurvivalEndothelial CellsProto-Oncogene Proteins c-aktSignal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1AnimalsMaleMiceMice, Inbred C57BLMuscle, SkeletalSatellite Cells, Skeletal MuscleAkt1 protein, mouseFlt1 protein, mouseProto-Oncogene Proteins c-aktVascular Endothelial Growth Factor Avascular endothelial growth factor A, mouseVascular Endothelial Growth Factor Receptor-1developmental biologyendothelial cellFlt1mousemuscle stem cellregenerative medicinesatellite cellskeletal musclestem cellsVEGF

Identifiers

PMID38842166
PMCPMC11216748

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.