Evidence map›Paper›PMID 38843496›Full record

ArticleBiomolecules & biomedicine2024

Puerarin alleviates osteoporosis in rats by targeting the JAK2/STAT3 signaling pathway.

Xinlei Zhao, Jiaxuan Zhou, Yanqing Liu, Jianguo Wang, Youcai Liu, Beiyu Wang, Caiting Han, Shengjie Zhao, Yijun Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules & biomedicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xinlei ZhaoBaotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China; Department of Orthopaedic Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China.
Jiaxuan ZhouBaotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China; Department of Orthopaedic Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China.
Yanqing LiuDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China.
Jianguo WangDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China.
Youcai LiuDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China.
Beiyu WangBaotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China; Department of Orthopaedic Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China.
Caiting HanBaotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China.
Shengjie ZhaoBaotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China.
Yijun ZhangDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, The Inner Mongolia Autonomous Region, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Osteoporosis (OP) is a common chronic progressive bone disease that increases fracture risk in postmenopausal women. Research suggests that puerarin (Pue) may be an effective treatment for OP. This study examined the effects and underlying mechanisms of Pue in treating postmenopausal osteoporosis (PMOP) in rats. Sprague-Dawley (SD) rats underwent bilateral ovariectomy to simulate PMOP and were then treated with subcutaneous injections of Pue. Bone mineral density (BMD) was measured using a bone densitometer. Micro-CT scans assessed femur bone structure and various parameters were calculated: bone volume fraction (BV/TV), bone surface density (BS/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp), and bone surface area-to-bone volume ratio (BS/BV). Hematoxylin-eosin (HE) staining was employed to observe femoral tissue pathology. Serum levels of bone formation metabolism-related markers-osteocalcin (OC), bone alkaline phosphatase (BALP), and procollagen type I N-terminal propeptide (PINP)-were measured via enzyme-linked immunosorbent assay (ELISA). The protein expression levels of the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway in bone tissue were evaluated using Western blotting assay. The results showed improved bone density and reduced bone loss in rats treated with Pue. There were also significant increases in serum levels of OC and BALP, indicating enhanced osteogenesis. Furthermore, there was a decrease in activation of the JAK2/STAT3 pathway in femoral tissue, suggesting a pathway inhibition. These findings indicate that Pue may combat osteoporosis by promoting osteogenesis and inhibiting activation of the JAK2/STAT3 pathway activation.

Indexed as

Bone DensityIsoflavonesJanus Kinase 2Rats, Sprague-DawleySignal TransductionSTAT3 Transcription FactorAnimalsFemaleFemurHumansOsteogenesisOsteoporosisOsteoporosis, PostmenopausalOvariectomyRatsX-Ray MicrotomographyIsoflavonesJak2 protein, ratJanus Kinase 2puerarinStat3 protein, ratSTAT3 Transcription Factor

Identifiers

PMID38843496
PMCPMC11496846

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.