Evidence map›Paper›PMID 38844174›Full record

ArticleBioorganic & medicinal chemistry letters2024

Extended voltage imaging in cardiomyocytes with a triplet state quencher-stabilized silicon rhodamine.

Kayli N Martinez, Nels C Gerstner, Samantha J Yang, Evan W Miller

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioorganic & medicinal chemistry letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Water-Soluble Molecular Wires for Membrane Potential Imaging.Angewandte Chemie (International ed. in English) · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kayli N MartinezDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720-1460, USA.
Nels C GerstnerDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720-1460, USA.
Samantha J YangDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720-1460, USA.
Evan W MillerDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720-1460, USA; Department of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA 94720-1460, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720-1460, USA. Electronic address: evanwmiller@berkeley.edu.

Funding

Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
New Chemical Tools for Exploring Cellular PhysiologyR35GM119855 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MILLER, EVAN WALKER · 2016 to 2020
$1.3M
Chemical Approaches to Visualize and Quantify Cellular PhysiologyR35GM153237 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Evan Walker Miller · 2024 to 2026
$1.3M
Design, synthesis and application of long wavelength fluorophores for bioimagingF32GM139263 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI GERSTNER, NELS · 2021 to 2022
$134k
NIGMS NIH HHS F32 GM139263NIGMS NIH HHS R35 GM119855NIGMS NIH HHS R35 GM153237NIGMS NIH HHS T32 GM066698
6 · The paper itself

Abstract

Voltage imaging of cardiac electrophysiology with voltage-sensitive dyes has long been a powerful complement to traditional methods like patch-clamp electrophysiology. Chemically synthesized voltage sensitive fluorophores offer flexibility for imaging in sensitive samples like human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs), since they do not require genetic transformation of the sample. One serious concern for any fluorescent voltage indicator, whether chemically synthesized or genetically encoded, is phototoxicity. We have been exploring self-healing fluorophores that use triplet state quenchers (TSQs) as a means to reduce the already low phototoxicity of VoltageFluor dyes developed in our lab. We previously showed that conjugation of the TSQ cyclooctatetraene (COT) to a fluorescein based VoltageFluor dye substantially reduced phototoxicity. Here, we show that this approach can be applied to far-red Silicon rhodamine dyes. COT-conjugated Si-rhodamines show improved photostability and reduced phototoxicity in hiPSC-CMs compared to the unmodified dye. This enables imaging of hiPSC-CMs for up to 30 min with continuous illumination. We show that this effect is mediated by a combination of reduced singlet oxygen production and lower loading in the cellular membrane. We discuss future applications and avenues of improvement for TSQ-stabilized VoltageFluor dyes.

Indexed as

Fluorescent DyesInduced Pluripotent Stem CellsMyocytes, CardiacRhodaminesHumansMolecular StructureSiliconFluorescent DyesRhodaminesSiliconFluorescence microscopyTriplet-state quenchersVoltage imaging

Identifiers

PMID38844174
PMCPMC11648968

What Socratic holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.