Evidence mapPaperPMID 38844340Full record

ArticleJournal of neurology, neurosurgery, and psychiatry2024

CSF sphingolipids are correlated with neuroinflammatory cytokines and differentiate neuromyelitis optica spectrum disorder from multiple sclerosis.

Lisa Shi, Laura Ghezzi, Chiara Fenoglio, Anna Margherita Pietroboni, Daniela Galimberti, Francesca Pace, Todd A Hardy, Laura Piccio, Anthony S Don

Abstract read
In one paragraph

Article in Journal of neurology, neurosurgery, and psychiatry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lisa Shi *School of Medical Sciences, Charles Perkins Centre, and Brain and Mind Centre, The University of Sydney, Sydney, New South Wales, Australia.
Laura Ghezzi *Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
Chiara FenoglioDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.
Anna Margherita PietroboniLa Fondazione IRCCS Ospedale Maggiore Policlinico, Milano, Italy.
Daniela GalimbertiDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy.ORCID http://orcid.org/0000-0002-9284-5953
Francesca PaceDepartment of Neurology, Washington University in St Louis, St Louis, Missouri, USA.
Todd A HardyConcord Hospital, Department of Neurology, The University of Sydney, Sydney, New South Wales, Australia.ORCID http://orcid.org/0000-0003-4145-3172
Laura PiccioSchool of Medical Sciences, Charles Perkins Centre, and Brain and Mind Centre, The University of Sydney, Sydney, New South Wales, Australia anthony.don@sydney.edu.au laura.piccio@sydney.edu.au.ORCID http://orcid.org/0000-0002-8760-109X
Anthony S DonSchool of Medical Sciences, Charles Perkins Centre, and Brain and Mind Centre, The University of Sydney, Sydney, New South Wales, Australia anthony.don@sydney.edu.au laura.piccio@sydney.edu.au.ORCID http://orcid.org/0000-0003-1655-1184

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThere is a need for biomarkers of disease progression and therapeutic response in multiple sclerosis (MS). This study aimed to identify cerebrospinal fluid (CSF) lipids that differentiate MS from other neuroinflammatory conditions and correlate with Expanded Disability Status Scale (EDSS) scores, gadolinium-enhancing lesions or inflammatory mediators.

methodsLipids and inflammatory cytokines/chemokines were quantified with liquid chromatography-tandem mass spectrometry and multiplex ELISA, respectively, in CSF from people with untreated MS, neuromyelitis optica spectrum disorder (NMOSD), other inflammatory neurological diseases and non-inflammatory neurological diseases (NIND). Analytes were compared between groups using analysis of variance, and correlations were assessed with Pearson's analysis.

resultsTwenty-five sphingolipids and four lysophosphatidylcholines were significantly higher in NMOSD compared with MS and NIND cases, whereas no lipids differed significantly between MS and NIND. A combination of three sphingolipids differentiated NMOSD from MS with the area under the curve of 0.92 in random forest models. Ninety-four lipids, including those that differentiated NMOSD from MS, were positively correlated with macrophage migration inhibitory factor (MIF) and 37 lipids were positively correlated with CSF protein in two independent MS cohorts. EDSS was inversely correlated with cholesterol ester CE(16:0) in both MS cohorts. In contrast, MIF and soluble triggering receptor expressed on myeloid cells 2 were positively associated with EDSS.

conclusionsCSF sphingolipids are positively correlated with markers of neuroinflammation and differentiate NMOSD from MS. The inverse correlation between EDSS and CE(16:0) levels may reflect poor clearance of cholesterol released during myelin break-down and warrants further investigation as a biomarker of therapeutic response.

Indexed as

BiomarkersCytokinesMultiple SclerosisNeuromyelitis OpticaSphingolipidsAdultDiagnosis, DifferentialFemaleHumansIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMaleMiddle AgedNeuroinflammatory DiseasesBiomarkersCytokinesIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMIF protein, humanSphingolipidsBIOCHEMISTRYCSFMULTIPLE SCLEROSIS

Identifiers

PMID38844340
PMCPMC11672031

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.