Evidence map›Paper›PMID 38858680›Full record

ArticleJournal of animal science and biotechnology2024

UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1.

Shengjie Shi, Huan Yuan, Lutong Zhang, Lei Gao, Lili Zhao, Xiangfang Zeng, Shiyan Qiao, Guiyan Chu, Chuanjiang Cai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shengjie ShiCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Huan YuanCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Lutong ZhangCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Lei GaoCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Lili ZhaoCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Xiangfang ZengState Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Center, China Agricultural University, Beijing, 100193, China.
Shiyan QiaoState Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Center, China Agricultural University, Beijing, 100193, China.
Guiyan ChuCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. guiyanchu@nwafu.edu.cn.ORCID http://orcid.org/0000-0001-9449-3894
Chuanjiang CaiCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. caichj@nwafu.edu.cn.

Funding

National Key R & D Program of China 2022YFD1300303National Natural Science Foundation of China 32272849
6 · The paper itself

Abstract

backgroundThe proliferation of porcine ovarian granulosa cells (GCs) is essential to follicular development and the ubiquitin-proteasome system is necessary for maintaining cell cycle homeostasis. Previous studies found that the deubiquitinase ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) regulates female reproduction, especially in ovarian development. However, the mechanism by which UCHL1 regulates porcine GC proliferation remains unclear.

resultsUCHL1 overexpression promoted GC proliferation, and knockdown had the opposite effect. UCHL1 is directly bound to cyclin B1 (CCNB1), prolonging the half-life of CCNB1 and inhibiting its degradation, thereby promoting GC proliferation. What's more, a flavonoid compound-isovitexin improved the enzyme activity of UCHL1 and promoted the proliferation of porcine GCs.

conclusionsUCHL1 promoted the proliferation of porcine GCs by stabilizing CCNB1, and isovitexin enhanced the enzyme activity of UCHL1. These findings reveal the role of UCHL1 and the potential of isovitexin in regulating proliferation and provide insights into identifying molecular markers and nutrients that affect follicle development.

Indexed as

CCNB1Granulosa cellsIsovitexinProliferationUCHL1

Identifiers

PMID38858680
PMCPMC11165742

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.