Evidence map›Paper›PMID 38863000›Full record

ArticleMobile DNA2024

Internal initiation of reverse transcription in a Penelope-like retrotransposon.

Chris J Frangieh, Max E Wilkinson, Daniel Strebinger, Jonathan Strecker, Michelle L Walsh, Guilhem Faure, Irina A Yushenova, Rhiannon K Macrae, Irina R Arkhipova, Feng Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mobile DNA, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chris J FrangiehHoward Hughes Medical Institute, Cambridge, MA, 02139, USA.
Max E WilkinsonHoward Hughes Medical Institute, Cambridge, MA, 02139, USA.
Daniel StrebingerHoward Hughes Medical Institute, Cambridge, MA, 02139, USA.
Jonathan StreckerHoward Hughes Medical Institute, Cambridge, MA, 02139, USA.
Michelle L WalshHoward Hughes Medical Institute, Cambridge, MA, 02139, USA.
Guilhem FaureHoward Hughes Medical Institute, Cambridge, MA, 02139, USA.
Irina A YushenovaJosephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA, 02543, USA.
Rhiannon K MacraeHoward Hughes Medical Institute, Cambridge, MA, 02139, USA.
Irina R ArkhipovaJosephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA, 02543, USA. iarkhipova@mbl.edu.
Feng ZhangHoward Hughes Medical Institute, Cambridge, MA, 02139, USA. zhang@broadinstitute.org.

Funding

National Science Foundation NSF MCB-2139001
6 · The paper itself

Abstract

Eukaryotic retroelements are generally divided into two classes: long terminal repeat (LTR) retrotransposons and non-LTR retrotransposons. A third class of eukaryotic retroelement, the Penelope-like elements (PLEs), has been well-characterized bioinformatically, but relatively little is known about the transposition mechanism of these elements. PLEs share some features with the R2 retrotransposon from Bombyx mori, which uses a target-primed reverse transcription (TPRT) mechanism, but their distinct phylogeny suggests PLEs may utilize a novel mechanism of mobilization. Using protein purified from E. coli, we report unique in vitro properties of a PLE from the green anole (Anolis carolinensis), revealing mechanistic aspects not shared by other retrotransposons. We found that reverse transcription is initiated at two adjacent sites within the transposon RNA that is not homologous to the cleaved DNA, a feature that is reflected in the genomic "tail" signature shared between and unique to PLEs. Our results for the first active PLE in vitro provide a starting point for understanding PLE mobilization and biology.

Indexed as

GIY-YIG endonucleaseHammerhead ribozymeReverse transcriptase

Identifiers

PMID38863000
PMCPMC11167929

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.