ArticleThe Plant cell2024
The MdHSC70-MdWRKY75 module mediates basal apple thermotolerance by regulating the expression of heat shock factor genes.
Article in The Plant cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The MdSLR1b-MdKNOX15-MdGA20ox2/MdGA2ox7 module regulates dwarfing by mediating the gibberellin pathway in apple.The Plant journal : for cell and molecular biology · 2026Article
- SnRK2.10 in apple positively regulates the nitrogen use efficiency via modulating the nitrogen uptake.The Plant journal : for cell and molecular biology · 2026Article
- Advances in Physiological and Molecular Mechanisms of Heat Stress in Apple and Pear.Plants (Basel, Switzerland) · 2026Review
- A VvWRKY8-salicylic acid pathway amplification loop confers resistance to Plasmopara viticola in grapevine.Molecular horticulture · 2026Article
- The MdERF1B-MdWRKY75-MdSOS3 Module Confers Salt Tolerance by Regulating Sodium-Potassium Homoeostasis in Apple.Plant, cell & environment · 2026Article
- Functional Characterization and Potential Regulatory Role ofInternational journal of molecular sciences · 2026Article
- Cold-responsive interaction between MdRAD23D1 and MdMYB15 confers cold stress tolerance via the CBF pathway in apple (Malus domestica).PLoS genetics · 2026Article
- The MdICE1/MdFAMA-MdTYDC Transcriptional Module Confers Cold Tolerance by Regulating Dopamine Metabolism in Apple.Plant biotechnology journal · 2026Article
- Genome-wide analysis of HSP70 family in Zygophyllum xanthoxylum and its heat stress response in Arabidopsis.Plant cell reports · 2026Article
- MaHSFA2c modulates high temperature-inhibited chlorophyll breakdown in banana fruit by directly inducing the transcription of the E3 ligase gene MaBAH1.Molecular horticulture · 2026Article
- Identification of the Auxin Response Factor gene family in kernel consumption apricot and association analysis of related genes regulating fruit size in distant hybridization.BMC plant biology · 2026Article
- Article
- The MdHB7L-MdICE1L-MdHOS1 Module Fine-Tunes Apple Cold Response via CBF-Dependent and CBF-Independent Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genome-wide identification of the apple NLP (NIN-like proteins) family and their potential role under abiotic stress.3 Biotech · 2025Article
- Transcriptome Analysis of Apple Leaves with Apple Necrotic Mosaic Virus-Associated Mosaic Symptoms.Plants (Basel, Switzerland) · 2025Article
- Betacyanin accumulation mediates photosynthetic protection in Suaeda salsa (L.) Pall. under salt stress.Planta · 2025Article
- Tissue-Specific RNA-Seq Analysis of Cotton Roots' Response to Compound Saline-Alkali Stress and the Functional Validation of the Key GenePlants (Basel, Switzerland) · 2025Article
- WRKY transcription factors participate in abiotic stress responses mediated by sugar metabolism.Frontiers in plant science · 2025Review
- Identification, characterization, and expression analysis of WRKY transcription factors inFrontiers in plant science · 2025Article
- Article
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11 authors.
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Abstract
Heat stress severely restricts the growth and fruit development of apple (Malus domestica). Little is known about the involvement of WRKY proteins in the heat tolerance mechanism in apple. In this study, we found that the apple transcription factor (TF) MdWRKY75 responds to heat and positively regulates basal thermotolerance. Apple plants that overexpressed MdWRKY75 were more tolerant to heat stress while silencing MdWRKY75 caused the opposite phenotype. RNA-seq and reverse transcription quantitative PCR showed that heat shock factor genes (MdHsfs) could be the potential targets of MdWRKY75. Electrophoretic mobility shift, yeast one-hybrid, β-glucuronidase, and dual-luciferase assays showed that MdWRKY75 can bind to the promoters of MdHsf4, MdHsfB2a, and MdHsfA1d and activate their expression. Apple plants that overexpressed MdHsf4, MdHsfB2a, and MdHsfA1d exhibited heat tolerance and rescued the heat-sensitive phenotype of MdWRKY75-Ri3. In addition, apple heat shock cognate 70 (MdHSC70) interacts with MdWRKY75, as shown by yeast two-hybrid, split luciferase, bimolecular fluorescence complementation, and pull-down assays. MdHSC70 acts as a negative regulator of the heat stress response. Apple plants that overexpressed MdHSC70 were sensitive to heat, while virus-induced gene silencing of MdHSC70 enhanced heat tolerance. Additional research showed that MdHSC70 exhibits heat sensitivity by interacting with MdWRKY75 and inhibiting MdHsfs expression. In summary, we proposed a mechanism for the response of apple to heat that is mediated by the "MdHSC70/MdWRKY75-MdHsfs" molecular module, which enhances our understanding of apple thermotolerance regulated by WRKY TFs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.