Evidence map›Paper›PMID 38867199›Full record

ArticleBMC complementary medicine and therapies2024

Kigelia africana fruit fractions inhibit in vitro alpha-glucosidase activity: a potential natural alpha-glucosidase inhibitor.

Tumelo Akapelwa Muyenga, Samuel K Dominion Bamitale, Dan Kibuule, Simbarashe Sithole, Stanley Mukanganyama, Carlen Rudolph, Luanne Venables, Anna C Hattingh, Maryna van de Venter, Christian Chinyere Ezeala

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Article in BMC complementary medicine and therapies, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

10 authors.

Tumelo Akapelwa MuyengaDepartment of Physiological Sciences, School of Medicine and Health Sciences, Mulungushi University, P.O. Box 60009, Livingstone, Zambia. tumelo.muyenga@mu.ac.zm.
Samuel K Dominion BamitaleDepartment of Internal Medicine and Pharmacology, Faculty of Medicine, and Health Sciences, Eastern Cape, Walter Sisulu University, Mthatha, South Africa.
Dan KibuuleDepartment of Pharmacology and Therapeutics, Faculty of Health Sciences, Busitema University, Busitema, Uganda.
Simbarashe SitholeDepartment of Chemistry and Earth Sciences, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe.
Stanley MukanganyamaDepartment of Biotechnology and Biotechnology, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe.
Carlen RudolphDepartment of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, South Africa.
Luanne VenablesDepartment of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, South Africa.
Anna C HattinghDepartment of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, South Africa.
Maryna van de VenterDepartment of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, South Africa.
Christian Chinyere EzealaCollege of Health Agriculture and Natural Sciences, Africa University, Mutare, Zimbabwe.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDiabetes affects 75% of people in low-income countries, where conventional drugs like metformin are available, but newer drugs like alpha-glucosidase inhibitors are not accessible to most Southern African patients.

aimTo evaluate the α-glucosidase and α-amylase inhibitory activities of fractionated aqueous extracts of Kigelia africana fruit (KAFE) and their phytochemical fingerprints using gas chromatography-mass spectrometry (GC-MS). MATERIALS AND

methodsWe studied K. africana fruit fractions' inhibitory effects on alpha-glucosidase and alpha-amylase using bioassay-guided fractionation, and analyzed their phytochemical profiles with GC-MS. KEY

findingsBoth the aqueous extract and ethyl acetate fraction of the aqueous extract exhibited a low dose-dependent inhibition of alpha-amylase activity (p < 0.0001). At a concentration of 500 μg/mL, the aqueous extract caused an alpha-glucosidase inhibition of 64.10 ± 2.7%, with an estimated IC50 of 193.7 μg/mL, while the ethyl acetate fraction had an inhibition of 89.82 ± 0.8% and an estimated IC50 of 10.41 μg/mL. The subfraction G, which had the highest alpha-glucosidase inhibitory activity at 85.10 ± 0.7%, had significantly lower activity than the ethyl acetate fraction. The most bioactive fraction was found to contain 11"(2-cyclopenten-1-yl) undecanoic acid, ( +)- and cyclopentane undecanoic acid as well as the indole alkaloids Akuammilan-17-ol-10-methoxy, N-nitroso-2-methyl-oxazolidine and epoxide Oxirane2.2″ -(1.4-butanediyl) bis-.

conclusionThe K. africana fruit fraction demonstrated significant alpha-glucosidase inhibitory activity, while its alpha-amylase inhibitory activity was limited. This study suggests a potential natural alpha-glucosidase inhibitor and phytocompounds that could serve as leads for developing antidiabetic agents.

Indexed as

FruitGlycoside Hydrolase InhibitorsPlant Extractsalpha-Amylasesalpha-GlucosidasesGas Chromatography-Mass SpectrometryHumansPhytochemicalsalpha-Amylasesalpha-GlucosidasesGlycoside Hydrolase InhibitorsPhytochemicalsPlant ExtractsAlpha-amylase inhibitionAlpha-glucosidase inhibitionDiabetesGas Chromatography- mass spectrometryKigelia africana fruit fractionsPhytochemistry

Identifiers

PMID38867199
PMCPMC11167833

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