Evidence map›Paper›PMID 38867795›Full record

ArticleBiomedical optics express2024

Triggered cagedSTORM microscopy.

Péter Bíró, Tibor Novák, Elvira Czvik, József Mihály, Szilárd Szikora, Sebastian van de Linde, Miklós Erdélyi

Abstract read
In one paragraph

Article in Biomedical optics express, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Péter BíróDepartment of Optics and Quantum Electronics, University of Szeged, Dóm tér 9, Szeged 6720, Hungary.ORCID https://orcid.org/0000-0001-8103-6248
Tibor NovákDepartment of Optics and Quantum Electronics, University of Szeged, Dóm tér 9, Szeged 6720, Hungary.
Elvira CzvikDepartment of Optics and Quantum Electronics, University of Szeged, Dóm tér 9, Szeged 6720, Hungary.
József MihályInstitute of Genetics, HUN-REN Biological Research Centre Szeged, Temesvári körút 62, Szeged 6726, Hungary.
Szilárd SzikoraInstitute of Genetics, HUN-REN Biological Research Centre Szeged, Temesvári körút 62, Szeged 6726, Hungary.
Sebastian van de LindeDepartment of Physics, SUPA, University of Strathclyde, Glasgow G4 0NG, Scotland, United Kingdom.
Miklós ErdélyiDepartment of Optics and Quantum Electronics, University of Szeged, Dóm tér 9, Szeged 6720, Hungary.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In standard SMLM methods, the photoswitching of single fluorescent molecules and the data acquisition processes are independent, which leads to the detection of single molecule blinking events on several consecutive frames. This mismatch results in several data points with reduced localization precision, and it also increases the possibilities of overlapping. Here we discuss how the synchronization of the fluorophores' ON state to the camera exposure time increases the average intensity of the captured point spread functions and hence improves the localization precision. Simulations and theoretical results show that such synchronization leads to fewer localizations with 15% higher sum signal on average, while reducing the probability of overlaps by 10%.

Identifiers

PMID38867795
PMCPMC11166440

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.