Evidence map›Paper›PMID 38868948›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2024

Dissecting Acute Drug-Induced Hepatotoxicity and Therapeutic Responses of Steatotic Liver Disease Using Primary Mouse Liver and Blood Cells in a Liver-On-A-Chip Model.

Hanyang Liu, Guo Yin, Marlene Sophia Kohlhepp, Fabian Schumacher, Jana Hundertmark, Mohamed I Abdelwahab Hassan, Felix Heymann, Tobias Puengel, Burkhard Kleuser, Alexander Sandy Mosig and 2 more

Abstract read
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

  1. Article
  2. Review
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  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Emerging nanomedicine for liver diseases treatment.Journal of nanobiotechnology · 2025
    Review
  12. Spatiotemporal liver dynamics: How far can we take themLiver research (Beijing, China) · 2025
    Article
  13. [Research progress on brucellosis combined with liver injury].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2025
    Review
  14. Review
  15. Article
  16. Review
  17. Review
  18. Article
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  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hanyang LiuDepartment of Hepatology & Gastroenterology, Campus Virchow-Klinikum and Campus Charité Mitte, Charité - Universitätsmedizin Berlin, 13353, Berlin, Germany.
Guo YinDepartment of Hepatology & Gastroenterology, Campus Virchow-Klinikum and Campus Charité Mitte, Charité - Universitätsmedizin Berlin, 13353, Berlin, Germany.
Marlene Sophia KohlheppDepartment of Hepatology & Gastroenterology, Campus Virchow-Klinikum and Campus Charité Mitte, Charité - Universitätsmedizin Berlin, 13353, Berlin, Germany.
Fabian SchumacherInstitute of Pharmacy, Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany.
Jana HundertmarkDepartment of Hepatology & Gastroenterology, Campus Virchow-Klinikum and Campus Charité Mitte, Charité - Universitätsmedizin Berlin, 13353, Berlin, Germany.
Mohamed I Abdelwahab HassanInstitute of Biochemistry II, Center for Sepsis Control and Care, Jena University Hospital, 07747, Jena, Germany.
Felix HeymannDepartment of Hepatology & Gastroenterology, Campus Virchow-Klinikum and Campus Charité Mitte, Charité - Universitätsmedizin Berlin, 13353, Berlin, Germany.
Tobias PuengelDepartment of Hepatology & Gastroenterology, Campus Virchow-Klinikum and Campus Charité Mitte, Charité - Universitätsmedizin Berlin, 13353, Berlin, Germany.
Burkhard KleuserInstitute of Pharmacy, Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany.
Alexander Sandy MosigInstitute of Biochemistry II, Center for Sepsis Control and Care, Jena University Hospital, 07747, Jena, Germany.
Frank TackeDepartment of Hepatology & Gastroenterology, Campus Virchow-Klinikum and Campus Charité Mitte, Charité - Universitätsmedizin Berlin, 13353, Berlin, Germany.
Adrien GuillotDepartment of Hepatology & Gastroenterology, Campus Virchow-Klinikum and Campus Charité Mitte, Charité - Universitätsmedizin Berlin, 13353, Berlin, Germany.ORCID 0000-0002-6002-9986

Funding

Bundesministerium für Bildung und Forschung ImmunAvatar consortiumDeutsche Forschungsgemeinschaft DFGTa434/8-1Deutsche Forschungsgemeinschaft Project-ID403224013Deutsche Forschungsgemeinschaft SFB1382Deutsche Forschungsgemeinschaft SFB/TRR296
6 · The paper itself

Abstract

Metabolic dysfunction-associated steatotic liver disease (MASLD) is hallmarked by hepatic steatosis, cell injury, inflammation, and fibrosis. This study elaborates on a multicellular biochip-based liver sinusoid model to mimic MASLD pathomechanisms and investigate the therapeutic effects of drug candidates lanifibranor and resmetirom. Mouse liver primary hepatocytes, hepatic stellate cells, Kupffer cells, and endothelial cells are seeded in a dual-chamber biocompatible liver-on-a-chip (LoC). The LoC is then perfused with circulating immune cells (CICs). Acetaminophen (APAP) and free fatty acids (FFAs) treatment recapitulate acute drug-induced liver injury and MASLD, respectively. As a benchmark for the LoC, multiplex immunofluorescence on livers from APAP-injected and dietary MASLD-induced mice reveals characteristic changes on parenchymal and immune cell populations. APAP exposure induces cell death in the LoC, and increased inflammatory cytokine levels in the circulating perfusate. Under FFA stimulation, lipid accumulation, cellular damage, inflammatory secretome, and fibrogenesis are increased in the LoC, reflecting MASLD. Both injury conditions potentiate CIC migration from the perfusate to the LoC cellular layers. Lanifibranor prevents the onset of inflammation, while resmetirom decreases lipid accumulation in hepatocytes and increases the generation of FFA metabolites in the LoC. This study demonstrates the LoC potential for functional and molecular evaluation of liver disease drug candidates.

Indexed as

AcetaminophenChemical and Drug Induced Liver InjuryDisease Models, AnimalAnimalsChalconesFatty LiverHepatocytesLab-On-A-Chip DevicesLiverMiceMice, Inbred C57BLPropionates2-(2,6-dimethyl-4-(3-(4-(methylthio)phenyl)-3-oxo-1-propenyl)phenoxyl)-2-methylpropanoic acidAcetaminophenChalconesPropionatesfibrosisliver diseasesmacrophagesmicrofluidic biochipNAFLDNASHsteatohepatitis

Identifiers

PMID38868948
PMCPMC11321671

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.