Evidence mapPaperPMID 38874707Full record

ArticleMolecular biology reports2024

Mir-204-5p alleviates mitochondrial dysfunction by targeting IGFBP5 in diabetic cataract.

Jin Xie, Peng Chen, Shilan Mao, Xinyi Zang, Rui Cao, Wenhui Liu, Xiaolei Wang, Yunhai Dai

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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3 citing papers in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Jin XieState Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Eye Institute of Shandong First Medical University, Qingdao, China.
Peng ChenDepartment of Human Anatomy, Histology and Embryology, School of Basic Medicine, Qingdao University, Qingdao, China.
Shilan MaoShandong First Medical University, Jinan, China.
Xinyi ZangWeifang Medical University, Weifang, China.
Rui CaoState Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Eye Institute of Shandong First Medical University, Qingdao, China.
Wenhui LiuThe Affiliated Hospital of Qingdao University, Qingdao, China.
Xiaolei WangState Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Eye Institute of Shandong First Medical University, Qingdao, China. xiaoleishengji@163.com.
Yunhai DaiState Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Eye Institute of Shandong First Medical University, Qingdao, China. yunhaidai@163.com.

Funding

National Natural Science Foundation of China 81970782National Natural Science Foundation of China 82000851Natural Science Foundation of Shandong Province ZR2020QH144Qilu Health Outstanding Young Talents Program A0241
6 · The paper itself

Abstract

backgroundCataract contributes to visual impairment worldwide, and diabetes mellitus accelerates the formation and progression of cataract. Here we found that the expression level of miR-204-5p was diminished in the lens epithelium with anterior lens capsule of cataract patients compared to normal donors, and decreased more obviously in those of diabetic cataract (DC) patients. However, the contribution and mechanism of miR-204-5p during DC development remain elusive. METHODS AND

resultThe mitochondrial membrane potential (MMP) was reduced in the lens epithelium with anterior lens capsule of DC patients and the H2O2-induced human lens epithelial cell (HLEC) cataract model, suggesting impaired mitochondrial functional capacity. Consistently, miR-204-5p knockdown by the specific inhibitor also attenuated the MMP in HLECs. Using bioinformatics and a luciferase assay, further by immunofluorescence staining and Western blot, we identified IGFBP5, an insulin-like growth factor binding protein, as a direct target of miR-204-5p in HLECs. IGFBP5 expression was upregulated in the lens epithelium with anterior lens capsule of DC patients and in the HLEC cataract model, and IGFBP5 knockdown could reverse the mitochondrial dysfunction in the HLEC cataract model.

conclusionsOur results demonstrate that miR-204-5p maintains mitochondrial functional integrity through repressing IGFBP5, and reveal IGFBP5 may be a new therapeutic target and prognostic factor for DC.

Indexed as

CataractDiabetes ComplicationsEpithelial CellsInsulin-Like Growth Factor Binding Protein 5MicroRNAsMitochondriaFemaleHumansLens, CrystallineMaleMembrane Potential, MitochondrialMiddle AgedIGFBP5 protein, humanInsulin-Like Growth Factor Binding Protein 5MicroRNAsMIRN204 microRNA, humanDiabetic cataractHuman anterior lens capsuleHuman lens epithelial cellsIGFBP5MiR-204-5pMitochondrial functional capacity

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.