Evidence mapPaperPMID 38892001Full record

ArticleInternational journal of molecular sciences2024

Hemoglobin Binding to the Red Blood Cell (RBC) Membrane Is Associated with Decreased Cell Deformability.

Gregory Barshtein, Leonid Livshits, Alexander Gural, Dan Arbell, Refael Barkan, Ivana Pajic-Lijakovic, Saul Yedgar

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Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Gregory BarshteinDepartment of Biochemistry, The Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112001, Israel.ORCID 0000-0001-6588-7229
Leonid LivshitsRed Blood Cell Research Group, Vetsuisse Faculty, Institute of Veterinary Physiology, University of Zurich, 8057 Zürich, Switzerland.
Alexander GuralBlood Bank, Hadassah University Hospital, Jerusalem 9112001, Israel.
Dan ArbellPediatric Surgery, Hadassah University Hospital, Jerusalem 9112001, Israel.
Refael BarkanDepartment of Digital Medical Technologies, Holon Institute of Technology, Holon 5810201, Israel.
Ivana Pajic-LijakovicDepartment of Chemical Engineering, University of Belgrade, 11000 Belgrade, Serbia.ORCID 0000-0001-9663-6916
Saul YedgarDepartment of Biochemistry, The Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Funding

Ernst Göhner Stiftung, Zug NAFondation Botnar, Basel NAHebrew University internal grant 314100748Susanne & René Braginsky Stiftung, Zurich NA
6 · The paper itself

Abstract

The deformability of red blood cells (RBCs), expressing their ability to change their shape as a function of flow-induced shear stress, allows them to optimize oxygen delivery to the tissues and minimize their resistance to flow, especially in microcirculation. During physiological aging and blood storage, or under external stimulations, RBCs undergo metabolic and structural alterations, one of which is hemoglobin (Hb) redistribution between the cytosol and the membrane. Consequently, part of the Hb may attach to the cell membrane, and although this process is reversible, the increase in membrane-bound Hb (MBHb) can affect the cell's mechanical properties and deformability in particular. In the present study, we examined the correlation between the MBHb levels, determined by mass spectroscopy, and the cell deformability, determined by image analysis. Six hemoglobin subunits were found attached to the RBC membranes. The cell deformability was negatively correlated with the level of four subunits, with a highly significant inter-correlation between them. These data suggest that the decrease in RBC deformability results from Hb redistribution between the cytosol and the cell membrane and the respective Hb interaction with the cell membrane.

Indexed as

Erythrocyte DeformabilityErythrocyte MembraneHemoglobinsErythrocytesHumansProtein BindingHemoglobinshemoglobinmembrane-bound hemoglobinRBC deformabilityred blood cells

Identifiers

PMID38892001
PMCPMC11172562

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.