Evidence map›Paper›PMID 38895415›Full record

ArticlebioRxiv : the preprint server for biology2024

AGS3-based optogenetic GDI induces GPCR-independent Gβγ signaling and macrophage migration.

Waruna Thotamune, Sithurandi Ubeysinghe, Chathuri Rajarathna, Dinesh Kankanamge, Koshala Olupothage, Aditya Chandu, Bryan A Copits, Ajith Karunarathne

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Waruna ThotamuneDepartment of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.ORCID 0000-0001-8695-8575
Sithurandi UbeysingheDepartment of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.ORCID 0009-0008-4647-7572
Chathuri RajarathnaDepartment of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.
Dinesh KankanamgeWashington University Pain Center, Department of Anesthesiology, Washington University School of Medicine; St. Louis, MO 63110 USA.ORCID 0000-0001-9647-0258
Koshala OlupothageDepartment of Chemistry and Biochemistry, The University of Toledo, Toledo, OH 43606, USA.ORCID 0000-0003-0121-4943
Aditya ChanduDepartment of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.
Bryan A CopitsWashington University Pain Center, Department of Anesthesiology, Washington University School of Medicine; St. Louis, MO 63110 USA.ORCID 0000-0003-3732-890X
Ajith KarunarathneDepartment of Chemistry, Saint Louis University, Saint Louis, MO 63103, USA.ORCID 0000-0003-3136-2015

Funding

Next Generation Opto-GPCRs for Neuromodulatory ControlU01NS128537 · NINDS · UNIVERSITY OF WASHINGTON · PI Andre Berndt, Michael R. Bruchas · 2023 to 2026
$4.4M
Next-gen Opto-GPCRs: spatiotemporal simulation of neuromodulator signalingR01MH111520 · NIMH · WASHINGTON UNIVERSITY · PI BRUCHAS, MICHAEL R., SUNAHARA, ROGER K · 2016 to 2018
$3.2M
Trans-synaptic optical control of user-defined synaptic connectionsUF1NS133763 · NINDS · WASHINGTON UNIVERSITY · PI COPITS, BRYAN, O'NEILL, PATRICK ROSS · 2023 to 2023
$1.7M
Optical control of endogenous G protein Coupled Receptor and G Protein Signaling.R01GM140191 · NIGMS · UNIVERSITY OF TOLEDO · PI KARUNARATHNE, WELIVITIYA KANKANAMLAGE AJITH · 2021 to 2023
$1.0M
NIGMS NIH HHS R01 GM140191NIMH NIH HHS R01 MH111520NINDS NIH HHS U01 NS128537NINDS NIH HHS UF1 NS133763
6 · The paper itself

Abstract

G protein-coupled receptors (GPCRs) are efficient Guanine nucleotide exchange factors (GEFs) and exchange GDP to GTP on the Gα subunit of G protein heterotrimers in response to various extracellular stimuli, including neurotransmitters and light. GPCRs primarily broadcast signals through activated G proteins, GαGTP, and free Gβγ, and are major disease drivers. Evidence shows that the ambient low threshold signaling required for cells is likely supplemented by signaling regulators such as non-GPCR GEFs and Guanine nucleotide Dissociation Inhibitors (GDIs). Activators of G protein Signaling 3 (AGS3) are recognized as a GDI involved in multiple health and disease-related processes. Nevertheless, understanding of AGS3 is limited, and no significant information is available on its structure-function relationship or signaling regulation in living cells. Here, we employed

Indexed as

Activators of G protein Signaling 3GDIGPCRG proteinsMacrophage migrationOptogenetics

Identifiers

PMID38895415
PMCPMC11185739

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.