Evidence map›Paper›PMID 38903015›Full record

ArticleDisease models & mechanisms2024

Contribution of Streptococcus pseudopneumoniae and Streptococcus salivarius to vocal fold mucosal integrity and function.

Vlasta Lungova, Madhu Gowda, Jessica M Fernandez, Stephanie Bartley, Anumitha Venkatraman, Federico E Rey, Susan L Thibeault

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Disease models & mechanisms, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Microbiome and Communication Disorders: A Tutorial for Clinicians.Journal of speech, language, and hearing research : JSLHR · 2025
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Vlasta LungovaDepartment of Surgery, Division of Otolaryngology, University of Wisconsin-Madison, Madison, WI 53792, USA.ORCID 0000-0002-5723-3441
Madhu GowdaDepartment of Surgery, Division of Otolaryngology, University of Wisconsin-Madison, Madison, WI 53792, USA.
Jessica M FernandezDepartment of Surgery, Division of Otolaryngology, University of Wisconsin-Madison, Madison, WI 53792, USA.
Stephanie BartleyDepartment of Surgery, Division of Otolaryngology, University of Wisconsin-Madison, Madison, WI 53792, USA.
Anumitha VenkatramanDepartment of Surgery, Division of Otolaryngology, University of Wisconsin-Madison, Madison, WI 53792, USA.
Federico E ReyDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Susan L ThibeaultDepartment of Surgery, Division of Otolaryngology, University of Wisconsin-Madison, Madison, WI 53792, USA.ORCID 0000-0002-9046-4356

Funding

Engineering the Vocal Fold MucosaR01DC004336 · NIDCD · UNIVERSITY OF WISCONSIN-MADISON · PI Susan Lynn Thibeault · 2000 to 2026
$14.6M
Mechanisms of innate immune-microbial interactions in vocal fold InflammationR01DC012773 · NIDCD · UNIVERSITY OF WISCONSIN-MADISON · PI THIBEAULT, SUSAN LYNN · 2013 to 2022
$4.5M
NIDCD NIH HHS R01 04336NIDCD NIH HHS R01 DC004336NIDCD NIH HHS R01 DC012773NIH HHSNIH - NIDCD R01DC04336University of Wisconsin-Madison
6 · The paper itself

Abstract

Structural changes to the vocal fold (VF) epithelium, namely, loosened intercellular junctions, have been reported in VF benign lesions. The potential mechanisms responsible for the disruption of cell junctions do not address the contribution of resident microbial communities to this pathological phenomenon. In this study, we focused on determining the relationship between Streptococcus pseudopneumoniae (SP), a dominant bacterial species associated with benign lesions, and Streptococcus salivarius (SS), a commensal bacterium, with human VF epithelial cells in our three-dimensional model of the human VF mucosa. This experimental system enabled direct deposition of bacteria onto constructs at the air/liquid interface, allowing for the assessment of bacterium-host interactions at the cellular, molecular and ultrastructural levels. Our findings demonstrate that SP disrupts VF epithelial integrity and initiates inflammation via the exported products HtrA1 and pneumolysin. In contrast, SS attaches to the VF epithelium, reduces inflammation and induces Mmp2-mediated apical desquamation of infected cells to mitigate the impact of pathogens. In conclusion, this study highlights the complexity of microbial involvement in VF pathology and potential VF mucosal restoration in the presence of laryngeal commensals.

Indexed as

Streptococcus salivariusVocal CordsEpithelial CellsHumansInflammationMucous MembraneStreptococcus pneumoniaeCommensalsEpithelial integrityPathogenic bacteriaRemodelingVocal fold mucosa

Identifiers

PMID38903015
PMCPMC11273296

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.