ArticlebioRxiv : the preprint server for biology2024
Phollow: Visualizing Gut Bacteriophage Transmission within Microbial Communities and Living Animals.
Lizett Ortiz de Ora, Elizabeth T Wiles, Mirjam Zünd, Maria S Bañuelos, Nancy Haro-Ramirez, Diana S Suder, Naveena Ujagar, Julio Ayala Angulo, Calvin Trinh, Courtney Knitter and 3 more
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In one paragraphArticle in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
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2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
13 authors.
Lizett Ortiz de OraDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.ORCID 0000-0003-1662-5489 Elizabeth T WilesDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.ORCID 0000-0001-7269-7466 Mirjam ZündDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.ORCID 0000-0003-2443-9328 Maria S BañuelosDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.ORCID 0009-0008-0768-486X Nancy Haro-RamirezDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.
Diana S SuderDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.
Naveena UjagarDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.
Julio Ayala AnguloDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.
Calvin TrinhDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.
Courtney KnitterDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.
Shane GonenDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.ORCID 0000-0002-4829-3151 Dequina A NicholasDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.ORCID 0000-0003-4996-2190 Travis J WilesDepartment of Molecular Biology & Biochemistry, University of California, Irvine, California, USA.ORCID 0000-0002-5336-7565 Funding
Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI RICHARD A. VAN ETTEN · 1994 to 2026
$57.9MUncover Strategies Used By Biological SystemsP50GM076516 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI NIE, QING · 2007 to 2016
$25.1MConnecting the Spatiotemporal Organization of Gut Bacterial Communities to the Emergence and Spread of Antibiotic ResistanceDP2AI154420 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI WILES, TRAVIS J · 2021 to 2025
$2.3MBreaking barriers in CryoEM through computational protein designR35GM142797 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GONEN, SHANE · 2021 to 2025
$1.9MThe Role of Energy Balance in Gonadotrope and Reproductive FunctionR00HD098330 · NICHD · UNIVERSITY OF CALIFORNIA-IRVINE · PI NICHOLAS, DEQUINA ANGELINA · 2021 to 2023
$816kZeiss LSM 980 NLO Confocal Microscope with Airyscan2 for UCI Optical Biology CoreS10OD032327 · OD · UNIVERSITY OF CALIFORNIA-IRVINE · PI WARRIOR, RAHUL · 2022 to 2022
$600kNCI NIH HHS P30 CA062203NIAID NIH HHS DP2 AI154420NICHD NIH HHS R00 HD098330NIGMS NIH HHS P50 GM076516NIGMS NIH HHS R35 GM142797NIH HHS S10 OD032327
6 · The paper itselfAbstract
Bacterial viruses (known as "phages") shape the ecology and evolution of microbial communities, making them promising targets for microbiome engineering. However, knowledge of phage biology is constrained because it remains difficult to study phage transmission dynamics within multi-member communities and living animal hosts. We therefore created "Phollow": a live imaging-based approach for tracking phage replication and spread in situ with single-virion resolution. Combining Phollow with optically transparent zebrafish enabled us to directly visualize phage outbreaks within the vertebrate gut. We observed that virions can be rapidly taken up by intestinal tissues, including by enteroendocrine cells, and quickly disseminate to extraintestinal sites, including the liver and brain. Moreover, antibiotics trigger waves of interbacterial transmission leading to sudden shifts in spatial organization and composition of defined gut communities. Phollow ultimately empowers multiscale investigations connecting phage transmission to transkingdom interactions that have the potential to open new avenues for viral-based microbiome therapies.
Identifiers
PMID38915633
PMCPMC11195241
What Socratic holds
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