Evidence mapPaperPMID 38920587Full record

ArticleBiosensors2024

Rapid Microfluidic Immuno-Biosensor Detection System for the Point-of-Care Determination of High-Sensitivity Urinary C-Reactive Protein.

Szu-Jui Chen, Song-Yu Lu, Chin-Chung Tseng, Kuan-Hsun Huang, To-Lin Chen, Lung-Ming Fu

Abstract read
In one paragraph

Article in Biosensors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Szu-Jui ChenDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.ORCID 0000-0002-6406-9280
Song-Yu LuDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.ORCID 0009-0009-0807-5086
Chin-Chung TsengDivision of Nephrology, Department of Internal Medicine, National Cheng Kung University Hospital, Tainan 70101, Taiwan.ORCID 0000-0002-8445-5269
Kuan-Hsun HuangDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.ORCID 0000-0001-8304-7151
To-Lin ChenDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.ORCID 0009-0003-4165-0309
Lung-Ming FuDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.ORCID 0000-0002-5422-3811

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A microfluidic immuno-biosensor detection system consisting of a microfluidic spectrum chip and a micro-spectrometer detection device is presented for the rapid point-of-care (POC) detection and quantification of high-sensitivity C-reactive protein (hs-CRP) in urine. The detection process utilizes a highly specific enzyme-linked immunosorbent assay (ELISA) method, in which capture antibodies and detection antibodies are pre-deposited on the substrate of the microchip and used to form an immune complex with the target antigen. Horseradish peroxidase (HRP) is added as a marker enzyme, followed by a colorimetric reaction using 3,3',5,5'-tetramethylbenzidine (TMB). The absorbance values (a.u.) of the colorimetric reaction compounds are measured using a micro-spectrometer device and used to measure the corresponding hs-CRP concentration according to the pre-established calibration curve. It is shown that the hs-CRP concentration can be determined within 50 min. In addition, the system achieves recovery rates of 93.8-106.2% in blind water samples and 94.5-104.6% in artificial urine. The results showed that the CRP detection results of 41 urine samples from patients with chronic kidney disease (CKD) were highly consistent with the conventional homogeneous particle-enhanced turbidimetric immunoassay (PETIA) method's detection results (R

Indexed as

Biosensing TechniquesC-Reactive ProteinEnzyme-Linked Immunosorbent AssayPoint-of-Care SystemsColorimetryHumansLab-On-A-Chip DevicesMicrofluidicsC-Reactive ProteinCKDC-reactive proteinELISAmicrochipmicrofluidic

Identifiers

PMID38920587
PMCPMC11201708

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.