Evidence map›Paper›PMID 38935929›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2024

From Bioreactor to Bulk Rheology: Achieving Scalable Production of Highly Concentrated Circular DNA.

Wynter A Paiva, Somkene D Alakwe, Juexin Marfai, Madigan V Jennison-Henderson, Rachel A Achong, Tinotenda Duche, April A Weeks, Rae M Robertson-Anderson, Nathan J Oldenhuis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Assembling a True "Olympic Gel" From over 16 000 Combinatorial DNA Rings.Advanced materials (Deerfield Beach, Fla.) · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wynter A PaivaDepartment of Chemistry, College of Engineering and Physical Science, University of New Hampshire, 23 Academic Way, Parsons Hall, Durham, NH 03824, USA.
Somkene D AlakweDepartment of Chemistry, College of Engineering and Physical Science, University of New Hampshire, 23 Academic Way, Parsons Hall, Durham, NH 03824, USA.
Juexin MarfaiDepartment of Physics and Biophysics, College of Arts and Sciences, University of San Diego, Shiley Center for Science and Technology, 5998 Alcala Park, San Diego, CA, 92110, USA.
Madigan V Jennison-HendersonDepartment of Chemistry, College of Engineering and Physical Science, University of New Hampshire, 23 Academic Way, Parsons Hall, Durham, NH 03824, USA.
Rachel A AchongDepartment of Chemistry, College of Engineering and Physical Science, University of New Hampshire, 23 Academic Way, Parsons Hall, Durham, NH 03824, USA.
Tinotenda DucheDepartment of Chemistry, College of Engineering and Physical Science, University of New Hampshire, 23 Academic Way, Parsons Hall, Durham, NH 03824, USA.
April A WeeksDepartment of Chemistry, College of Engineering and Physical Science, University of New Hampshire, 23 Academic Way, Parsons Hall, Durham, NH 03824, USA.
Rae M Robertson-AndersonDepartment of Physics and Biophysics, College of Arts and Sciences, University of San Diego, Shiley Center for Science and Technology, 5998 Alcala Park, San Diego, CA, 92110, USA.
Nathan J OldenhuisDepartment of Chemistry, College of Engineering and Physical Science, University of New Hampshire, 23 Academic Way, Parsons Hall, Durham, NH 03824, USA.ORCID 0000-0003-4256-0826

Funding

TLR-TRIF mediated induction of GLI3 modulates innate inflammatory responsesP20GM113131 · NIGMS · UNIVERSITY OF NEW HAMPSHIRE · PI Sean Stoddart Coleman Edington · 2017 to 2026
$22.8M
Using DNA hydrogels to mimic, exploit, and fundamentally investigate extracellular matricesR35GM154998 · NIGMS · UNIVERSITY OF NEW HAMPSHIRE · PI Nathan John Oldenhuis · 2024 to 2026
$1.1M
Biomimetic cytoskeleton and advanced microscopy to reveal intracellular DNA dynamics and distributionsR15GM123420 · NIGMS · UNIVERSITY OF SAN DIEGO · PI MCGORTY, RYAN, ROBERTSON-ANDERSON, RAE MARIE · 2017 to 2022
$939k
Air Force Office of Scientific Research FA9550-21-1-0361National Science Foundation 1757371National Science Foundation 2340569NIGMS NIH HHS P20 GM113131NIGMS NIH HHS P20GM113131NIGMS NIH HHS R15 GM123420NIGMS NIH HHS R15GM123420NIGMS NIH HHS R35 GM154998
6 · The paper itself

Abstract

DNA serves as a model system in polymer physics due to its ability to be obtained as a uniform polymer with controllable topology and nonequilibrium behavior. Currently, a major obstacle in the widespread adoption of DNA is obtaining it on a scale and cost basis that accommodates bulk rheology and high-throughput screening. To address this, recent advancements in bioreactor-based plasmid DNA production is coupled with anion exchange chromatography producing a unified approach to generating gram-scale quantities of monodisperse DNA. With this method, 1.1 grams of DNA is obtained per batch to generate solutions with concentrations up to 116 mg mL

Indexed as

BioreactorsDNA, CircularPlasmidsRheologyDNA, Circularbioreactorscircular polymersplasmid DNArheologyunimolecular polymers

Identifiers

PMID38935929
PMCPMC11955860

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.