Evidence mapPaperPMID 38938373Full record

ArticleJournal of extracellular biology2023

Tetraspanin 15 depletion impairs extracellular vesicle docking at target neurons.

Daniele Stajano, Franco L Lombino, Michaela Schweizer, Markus Glatzel, Paul Saftig, Kira V Gromova, Matthias Kneussel

Erratum issuedAbstract read
In one paragraph

Article in Journal of extracellular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Tetraspanin TSP-12 and SUP-17/ADAM10 exhibit cell type-specific codependence for trafficking through the Golgi.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Daniele StajanoInstitute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany.
Franco L LombinoInstitute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany.
Michaela SchweizerCore Facility Morphology, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany.
Markus GlatzelInstitute of Neuropathology University Medical Center Hamburg-Eppendorf Hamburg Germany.ORCID https://orcid.org/0000-0002-7720-8817
Paul SaftigBiochemical Institute Christian-Albrechts-University Kiel Kiel Germany.
Kira V GromovaInstitute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany.
Matthias KneusselInstitute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH University Medical Center Hamburg-Eppendorf Hamburg Germany.ORCID https://orcid.org/0000-0003-4900-366X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neurons in the central nervous system release extracellular vesicles (EVs) and exosomes in response to synaptic activity to regulate physiological processes at target neurons. The intercellular transfer of proteins, mRNAs, lipids or metabolites through EVs potentially modulates the structure and function of neurons and circuits. Whereas the biogenesis of EVs, their release from donor cells, and their molecular composition have been studied extensively, the critical factors and mechanisms regulating EV interactions with target cells are incompletely understood. Here, we identified tetraspanin 15 (Tspan15) as a component of tumor susceptibility gene 101 protein (TSG101)- and CD81-positive EV fractions. Tspan15 fluorescent fusion proteins were released from donor cells and interacted with target cells together with the exosomal marker CD63. EVs collected from wildtype cortical neurons (WT-EVs) underwent similar association with target neurons derived from either wildtype (+/+) or Tspan15 knockout (-/-) mice. In contrast, target cell interactions of EVs collected from Tspan15 (-/-) cortical donor neurons (KO-EVs) were significantly impaired, as compared to WT-EVs. Our data suggest that Tspan15 is dispensable at target neuron plasma membranes, but is required at the EV surface to promote EV docking at target neurons.

Indexed as

dockingexosomeextracellular vesicleintercellular communicationneurontarget celltetraspanin

Identifiers

PMID38938373
PMCPMC11080857

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.