ArticleJournal of extracellular biology2023
Removal and identification of external protein corona members from RBC-derived extracellular vesicles by surface manipulating antimicrobial peptides.
Article in Journal of extracellular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Biomolecular Corona of Extracellular Vesicles: Report on Isev Workshop.Journal of extracellular biology · 2026Article
- Urinary vesicle biomarkers and kidney function-results from the German AugUR study.Scientific reports · 2026Article
- Impact of Isolation Techniques on the Content of Small Extracellular Vesicles.Journal of extracellular vesicles · 2026Article
- Engineering challenges and translational opportunities in emerging gene delivery platforms.Nature biomedical engineering · 2026Review
- Biomolecular Corona Remodelling ofJournal of extracellular biology · 2026Article
- Biology and therapeutic potential of extracellular vesicle targeting and uptake.Nature reviews. Molecular cell biology · 2026Review
- An ATP-Mediated Antibiotic β-Peptide Nanofiber That Kills Multidrug-Resistant Bacteria via a Multistage Mechanism.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- EVennCorona: Annotating Protein Corona of Extracellular Vesicles by Venn Diagram.Journal of extracellular biology · 2026Article
- Comparison of technologies for manufacturing extracellular vesicles for therapeutic applications.Trends in biotechnology · 2026Article
- Ultracentrifugation and Ultrafiltration Differentially Alter the Composition and Functionality of the Biomolecular Corona of Extracellular Vesicles.Journal of extracellular biology · 2026Article
- Residual flexibility in the topologically constrained multivalent complex between the GKAP scaffold and LC8 hub proteins.The FEBS journal · 2026Article
- Monitoring extracellular vesicle surface glyco-properties using fluorescent lectins and nanoparticle tracking analysis.Open biology · 2025Article
- Small Extracellular Vesicles Released from ARPE-19 Cells Grown under Diabetic Retinopathy Conditions Promote NLRP3 Inflammasome Activation.ACS biomaterials science & engineering · 2025Article
- Article
- The extracellular vesicle biomolecular corona: current insights and diagnostic potential.Nanomedicine (London, England) · 2025Review
- A protein corona modulates the function of mineralization-competent matrix vesicles.JBMR plus · 2025Article
- In situ captured antibacterial action of membrane-incising peptide lamellae.Nature communications · 2024Article
- Factors to consider before choosing EV labeling method for fluorescence-based techniques.Frontiers in bioengineering and biotechnology · 2024Review
- Removal and identification of external protein corona members from RBC-derived extracellular vesicles by surface manipulating antimicrobial peptides.Journal of extracellular biology · 2023Article
Corrections and comments
- Erratum issuedCorrection to2025
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the last years, extracellular vesicles (EVs), secreted by various cells and body fluids have shown extreme potential in biomedical applications. Increasing number of studies suggest that a protein corona could adhere to the surface of EVs which can have a fundamental effect on their function, targeting and therapeutical efficacy. However, removing and identifying these corona members is currently a challenging task to achieve. In this study we have employed red blood cell-derived extracellular vesicles (REVs) as a model system and three membrane active antimicrobial peptides (AMPs), LL-37, FK-16 and CM15, to test whether they can be used to remove protein corona members from the surface of vesicles. These AMPs were reported to preferentially exert their membrane-related activity via one of the common helical surface-covering models and do not significantly affect the interior of lipid bilayer bodies. The interaction between the peptides and the REVs was followed by biophysical techniques, such as flow-linear dichroism spectroscopy which provided the effective applicable peptide concentration for protein removal. REV samples were then subjected to subsequent size exclusion chromatography and to proteomics analysis. Based on the comparison of control REVs with the peptide treated samples, seventeen proteins were identified as external protein corona members. From the three investigated AMPs, FK-16 can be considered as the best candidate to further optimize EV-related applicability of AMPs. Our results on the REV model system envisage that membrane active peptides may become a useful set of tools in engineering and modifying surfaces of EVs and other lipid-based natural particles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.