ArticleClinical oral investigations2024
Biocompatibility, bioactivity and immunomodulatory properties of three calcium silicate-based sealers: an in vitro study on hPDLSCs.
Article in Clinical oral investigations, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Immunomodulatory and angiogenic strategies in vital pulp therapy: a systematic review of scaffold-based, scaffold-free, and cell-laden interventions.Clinical oral investigations · 2026Pooled it
- Comparative efficacy of novel biomaterials versus traditional materials in the treatment of dental and pulpal diseases: a systematic review and meta-analysis.Frontiers in medicine · 2026Pooled it
- Palm Mixed-Carotenes Modulate Viability, Wound Closure and Osteoprotegerin mRNA Expression in Human Periodontal Ligament Stem Cells.Biomedicines · 2026Article
- Calcium silicate cement with phosphate-based glass for enhanced osteogenic activity and mechanical strength.Clinical oral investigations · 2026Article
- Bioactivity and dimensional stability of resin-, hybrid-, and silicate-based endodontic sealers: An in vitro comparative study.Journal of clinical and experimental dentistry · 2026Review
- Oxidative Stress, Pro-Inflammatory Response, Cytotoxicity and Apoptosis Induced by Contemporary Endodontic Sealers in Human Periodontal Ligament Fibroblasts.Journal of functional biomaterials · 2026Article
- Physicochemical and biological properties of three calcium silicate-based root canal sealers.PloS one · 2026Article
- Osteogenic Potential of Various Premixed Hydraulic Calcium Silicate-Based Sealers on Human Bone Marrow Stem Cells.Materials (Basel, Switzerland) · 2025Article
- Studying How Calcium Silicate and Radiopacifier Proportions Affect the Physicochemical Properties of Endodontic Calcium Silicate-Based Sealers.Materials (Basel, Switzerland) · 2025Article
- Evaluation of osteoconductivity and inflammatory response of a resin-based and a resin-free calcium silicate sealer: an in vivo study.Clinical oral investigations · 2025Article
- Bioceramics in Endodontics: Limitations and Future Innovations-A Review.Dentistry journal · 2025Review
- Impact of different capping materials extracts on proliferation and osteogenic differentiation of cultured human dental pulp stem cells.Scientific reports · 2025Article
- Effect of Bio MTA plus & ProRoot MTA pulp capping materials on the regenerative properties of human dental pulp stem cells.Scientific reports · 2025Article
- Effects of 0.01 mM strontium on human periodontal ligament stem cell osteogenic differentiation via the Wnt/The Journal of international medical research · 2025Article
- Root canal cleanliness and debris extrusion following retreatment of thermoplastic injection technique and bioceramic-based root canal sealer.Clinical oral investigations · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo assess the biocompatibility, bioactivity, and immunomodulatory properties of three new calcium silicate cement-based sealers: Ceraseal (CS), Totalfill BC Sealer (TFbc) and WellRoot ST (WR-ST) on human periodontal ligament stem cells (hPDLSCs). MATERIALS AND
methodsHPDLSCs were isolated from extracted third molars from healthy patients. Eluates (1:1, 1:2, and 1:4 ratio) and sample discs of CS, TFbc and WR-ST after setting were prepared. A series of assays were performed: cell characterization, cell metabolic activity (MTT assay) cell attachment and morphology (SEM assay), cell migration (wound-healing assay), cytoskeleton organization (phaloidin-based assay); IL-6 and IL-8 release (ELISA); differentiation marker expression (RT-qPCR assay), and cell mineralization (Alizarin Red S staining). HPDLSCs cultured in unconditioned (negative control) or osteogenic (positive control) culture media were used as a comparison. Statistical significance was established at p < 0.05.
resultsAll the tested sealers exhibited similar results in the cytocompatibility assays (cell metabolic activity, migration, attachment, morphology, and cytoskeleton organization) compared with a negative control group. CS and TFbc exhibited an upregulation of at least one osteo/cementogenic marker compared to the negative and positive control groups. CS and TFbc also showed a significantly higher calcified nodule formation than the negative and positive control groups. Both the marker expression and calcified nodule formation were significantly higher in CS-treated cells than TFbc treated cells. WR-ST exhibited similar results to the control group. CS and TFbc-treated cells exhibited a significant downregulation of IL-6 after 72 h of culture compared to the negative control group (p < 0.05).
conclusionAll the tested sealers exhibited an adequate cytocompatibility. CS significantly enhances cell differentiation by upregulating the expression of key genes associated with bone and cementum formation. Additionally, CS was observed to facilitate the mineralization of the extracellular matrix effectively. In contrast, the effects of TFbc and WR-ST on these processes were less pronounced compared to CS. Furthermore, both CS and TFbc exhibited an anti-inflammatory potential, contributing to their potential therapeutic benefits in regenerative endodontics. CLINICAL RELEVANCE: This is the first study to compare the biological properties and immunomodulatory potential of Ceraseal, Totalfill BC Sealer, and WellRoot ST. The results act as supporting evidence for their use in root canal treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.