Evidence mapPaperPMID 38973003Full record

ArticleBMC cancer2024

Comparative immune profiling of pancreatic ductal adenocarcinoma progression among South African patients.

Nnenna Elebo, Ebtesam A Abdel-Shafy, Jones A O Omoshoro-Jones, Zanele Nsingwane, Ahmed A A Hussein, Martin Smith, Geoffrey Candy, Stefano Cacciatore, Pascaline Fru, Ekene Emmanuel Nweke

Abstract readComparative Study
In one paragraph

Article in BMC cancer, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nnenna EleboDepartment of Surgery, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, 2193, South Africa.
Ebtesam A Abdel-ShafyBioinformatics Unit, International Centre for Genetic Engineering and Biotechnology, Observatory, Cape Town, 7925, South Africa.
Jones A O Omoshoro-JonesDepartment of Surgery, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, 2193, South Africa.
Zanele NsingwaneDepartment of Surgery, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, 2193, South Africa.
Ahmed A A HusseinBioinformatics Unit, International Centre for Genetic Engineering and Biotechnology, Observatory, Cape Town, 7925, South Africa.
Martin SmithDepartment of Surgery, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, 2193, South Africa.
Geoffrey CandyDepartment of Surgery, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, 2193, South Africa.
Stefano CacciatoreBioinformatics Unit, International Centre for Genetic Engineering and Biotechnology, Observatory, Cape Town, 7925, South Africa.
Pascaline FruDepartment of Surgery, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, 2193, South Africa.
Ekene Emmanuel NwekeDepartment of Surgery, Faculty of Health Sciences, University of Witwatersrand, Johannesburg, 2193, South Africa. ekene.nweke@wits.ac.za.

Funding

Cancer Association of South Africa N/ANational Research Foundation 121277National Research Foundation 138367South African Medical Research Council N/A
6 · The paper itself

Abstract

backgroundPancreatic Ductal Adenocarcinoma (PDAC) is an aggressive cancer characterized by an immunosuppressive microenvironment. Patients from specific ethnicities and population groups have poorer prognoses than others. Therefore, a better understanding of the immune landscape in such groups is necessary for disease elucidation, predicting patient outcomes and therapeutic targeting. This study investigated the expression of circulating key immune cell markers in South African PDAC patients of African ancestry.

methodsBlood samples were obtained from a total of 6 healthy volunteers (HC), 6 Chronic Pancreatitis (CP) and 34 PDAC patients consisting of 22 resectable (RPC), 8 locally advanced (LAPC) and 4 metastatic (MPC). Real-time Quantitative Polymerase Chain reactions (RT-qPCR), Metabolomics, Enzyme-Linked Immunosorbent Assay (ELISA), Reactive Oxygen Species (ROS), and Immunophenotyping assays were conducted. Statistical analysis was conducted in R (v 4.3.2). Additional analysis of single-cell RNA data from 20 patients (16 PDAC and 4 controls) was conducted to interrogate the distribution of T-cell and Natural Killer cell populations.

resultsGranulocyte and neutrophil levels were significantly elevated while lymphocytes decreased with PDAC severity. The total percentages of CD3 T-cell subpopulations (helper and double negative T-cells) decreased when compared to HC. Although both NK (p = 0.014) and NKT (p < 0.001) cell levels increased as the disease progressed, their subsets: NK CD56

conclusionThe expression of these immune cell markers observed in this pilot study provides insight into their potential roles in tumour progression in the patient group and suggests their potential utility in the development of immunotherapeutic strategies.

Indexed as

Carcinoma, Pancreatic DuctalDisease ProgressionPancreatic NeoplasmsAdultAgedBiomarkers, TumorFemaleHumansImmunophenotypingKiller Cells, NaturalMaleMiddle AgedPancreatitis, ChronicReactive Oxygen SpeciesSouth AfricaBiomarkers, TumorReactive Oxygen SpeciesCD4CD8Immune cellsImmunosuppressionPancreatic ductal adenocarcinomaPDAC

Identifiers

PMID38973003
PMCPMC11229237

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