Evidence map›Paper›PMID 38976218›Full record

ArticleNeuroscience bulletin2024

STED Imaging of Vesicular Endocytosis in the Synapse.

Shaoqin Hu, Zhenli Xie, Bianbian Wang, Yang Chen, Zexin Jing, Ying Hao, Jingyu Yao, Xuanang Wu, Jingxiao Huo, Anqi Wei and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience bulletin, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Shaoqin Hu *Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Zhenli Xie *Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Bianbian Wang *Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Yang ChenNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Zexin JingNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Ying HaoNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Jingyu YaoNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.ORCID http://orcid.org/0009-0007-0911-1363
Xuanang WuNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Jingxiao HuoNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Anqi WeiNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.ORCID http://orcid.org/0000-0002-0285-2974
Yuhao QinKey Laboratory of Medical Electrophysiology, Ministry of Education of China, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, and the Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 646000, China.
Nan DongNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Chaowen ZhengNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Qian SongNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Jiangang LongNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China.
Xinjiang KangCollege of Life Sciences, Liaocheng University, Liaocheng, 252059, China. kxj335@163.com.ORCID http://orcid.org/0000-0003-0877-2438
Changhe WangNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China. changhewang@xjtu.edu.cn.ORCID http://orcid.org/0000-0002-5902-2277
Huadong XuNeuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Department of Neurology, the First Affiliated Hospital, Core Facilities Sharing Platform, Xi'an Jiaotong University, Xi'an, 710049, China. hdxu@pku.edu.cn.ORCID http://orcid.org/0009-0005-5033-0352

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endocytosis is a fundamental biological process that couples exocytosis to maintain the homeostasis of the plasma membrane and sustained neurotransmission. Super-resolution microscopy enables optical imaging of exocytosis and endocytosis in live cells and makes an essential contribution to understanding molecular mechanisms of endocytosis in neuronal somata and other types of cells. However, visualization of exo-endocytic events at the single vesicular level in a synapse with optical imaging remains a great challenge to reveal mechanisms governing the synaptic exo-endocytotic coupling. In this protocol, we describe the technical details of stimulated emission depletion (STED) imaging of synaptic endocytosis at the single-vesicle level, from sample preparation and microscopy calibration to data acquisition and analysis.

Indexed as

EndocytosisSynapsesSynaptic VesiclesAnimalsExocytosisMicroscopy, FluorescenceNeuronsEndocytosisSingle-vesicle levelSTED imagingSynaptic vesicle

Identifiers

PMID38976218
PMCPMC11365914

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.