Evidence map›Paper›PMID 38977585›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Optimized Lipidomics Extraction of Sphingosine and Sphinganine from Optic Nerve for Signaling Studies.

Alexa Jauregui, Emily J Neag, Amr Almobayed, Alexandra Lens, Sanjoy K Bhattacharya

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Alexa JaureguiBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Emily J NeagBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Amr AlmobayedBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Alexandra LensBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Sanjoy K BhattacharyaBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA. sbhattacharya@med.miami.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Interconvertible sphingolipid metabolites represent germane constituents of eukaryotic membranes and are vital in the regulation of cellular homeostasis, proliferation, survival, and induction of autophagy. This protocol describes a step-by-step method for extractions of sphingosine and sphinganine from mammalian tissue samples, particularly from the murine optic nerve. These lipids are partitioned into a binary mixture of chloroform and methanol in a modified Bligh and Dyer method. This is followed with reverse phase ultrahigh-performance liquid chromatography fractionation with a C18+ column and subsequent tandem mass spectrometry (UHPLC-MS-MS) analysis of the biological abundance. These free sphingoid bases dissociate to form structurally distinctive carbocation product ions that can be confirmed with annotations of lipidomic databases or in-house fragmentation software.

Indexed as

LipidomicsOptic NerveSphingosineTandem Mass SpectrometryAnimalsChromatography, High Pressure LiquidMiceSignal TransductionsafingolSphingosineBligh and DyerImaging mass spectrometryLipidomicsMass spectrometryOptic nerveRegeneration

Identifiers

PMID38977585

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.