Evidence map›Paper›PMID 38977591›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Effects of a Laparotomy on Targeted Lipidomics Profiles in a Mouse Model of Surgical Stress During Aging.

Jian Huang, Kamal Awad, Lauren Harlow, Carlos A Toro, Marco Brotto, Christopher P Cardozo

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jian HuangBone-Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, The University of Texas at Arlington, Arlington, TX, USA.
Kamal AwadBone-Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, The University of Texas at Arlington, Arlington, TX, USA.
Lauren HarlowSpinal Cord Damage Research Center, James J. Peters VA Medical Center, Bronx, NY, USA.
Carlos A ToroSpinal Cord Damage Research Center, James J. Peters VA Medical Center, Bronx, NY, USA.
Marco BrottoBone-Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, The University of Texas at Arlington, Arlington, TX, USA.
Christopher P CardozoSpinal Cord Damage Research Center, James J. Peters VA Medical Center, Bronx, NY, USA. christopher.cardozo@mssm.edu.

Funding

Transgenic and Mechanical Loading CoreP01AG039355 · NIA · UNIVERSITY OF MISSOURI KANSAS CITY · PI BONEWALD, LYNDA F · 2012 to 2024
$19.3M
Preserving Mitochondrial Function for Alleviating ALS ProgressionR01NS105621 · NINDS · UNIVERSITY OF TEXAS ARLINGTON · PI BROTTO, MARCO, ZHOU, JINGSONG · 2019 to 2023
$2.9M
Regulation of Store-Operated Calcium Entry During Muscle AgingR01AG056504 · NIA · OHIO STATE UNIVERSITY · PI BROTTO, MARCO, WEISLEDER, NOAH · 2018 to 2022
$2.6M
Loss of Numb in Muscle Dysfunction in AgingR01AG060341 · NIA · BRONX VETERANS MEDICAL RESEARCH FDN · PI BROTTO, MARCO, CARDOZO, CHRISTOPHER P · 2018 to 2022
$2.4M
Protecting the Diabetic Skeletal Muscle by Nampt ActivationR01DK119066 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI BROTTO, MARCO, TIPPARAJU, SRINIVAS M. · 2019 to 2023
$2.0M
Post-operative Muscle and Physical Decline in Aged Organism: Mechanisms and InterventionsK08AG050808 · NIA · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KO, FRED CHAU-YANG · 2015 to 2019
$626k
NIA NIH HHS K08 AG050808NIA NIH HHS P01 AG039355NIA NIH HHS R01 AG056504NIA NIH HHS R01 AG060341NIDDK NIH HHS R01 DK119066NINDS NIH HHS R01 NS105621
6 · The paper itself

Abstract

Laparotomy (EL) is one of the most common procedures performed among surgical specialties. Previous research demonstrates that surgery is associated with an increased inflammatory response. Low psoas muscle mass and quality markers are associated with increased mortality rates after emergency laparotomy. Analysis of lipid mediators in serum and muscle by using liquid chromatography-mass spectrometry (LC-MS)-based lipidomics has proven to be a sensitive and precise technique. In this chapter, we describe an LC-MS/MS protocol for the profiling and quantification of signaling lipids formed from Eicosapentaenoic Acid (EPA) and Eicosatetranoic acid (ETA) by 5, 12, or 15 lipoxynases. This protocol has been developed for and validated in serum and muscle samples in a mouse model of surgical stress caused by laparotomy.

Indexed as

AgingLaparotomyLipidomicsTandem Mass SpectrometryAnimalsChromatography, LiquidDisease Models, AnimalLipid MetabolismLipidsMiceStress, PhysiologicalLipidsLaparotomyLipidomicsMuscleOmicsSerumSurgical stress

Identifiers

PMID38977591

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.