Evidence map›Paper›PMID 38982003›Full record

ArticleNeurocritical care2024

Visualization of Blood-Brain Barrier Disruption in Septic Mice with the New Method Based on in Vivo Imaging Technology.

Haisong Zhang, Yuhang Ai, Xiaolei Zhang, Fuxing Deng, Shiwei Jiang, Shucai Xie, Milin Peng, Wei Chen, Jiyun Hu, Songyun Deng and 1 more

Abstract read
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Article in Neurocritical care, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Potential vascular confound in GPR84-PET imaging following systemic LPS administration.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Haisong ZhangDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Yuhang AiDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Xiaolei ZhangDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Fuxing DengDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Shiwei JiangDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Shucai XieDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Milin PengDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Wei ChenDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Jiyun HuDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Songyun DengDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
Lina ZhangDepartment of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. zln7095@163.com.ORCID 0000-0003-3763-0498

Funding

National Natural Science Foundation of China 81873956National Natural Science Foundation of China 82172145
6 · The paper itself

Abstract

backgroundDynamic monitoring of the blood-brain barrier (BBB) functional status in septic mice can help to explore the pathological mechanisms. Therefore, we proposed a new method for monitoring BBB permeability and applied it to the detection of sepsis models.

methodsThe new method involves the construction of an optical cranial window and in vivo imaging. We performed dynamic monitoring of BBB permeability and cerebral blood flow (CBF) in cecal ligation puncture (CLP) and endotoxemia (lipopolysaccharide [LPS]) mice.

resultsThe sensitivity and accuracy of this method were higher than those of Evans blue evaluation. The increase of BBB permeability in the group of CLP mice was relatively mild and correlated with overall survival, and the damage was irreversible. Contrarily, BBB damage in the LPS group was more acute and severe, unrelated to overall survival, but recoverable. The CBF decreased significantly in both model mouse groups 24 h after modeling, but only the CBF proportion decrease in the LPS group was significantly correlated with an increase in BBB permeability. Within 24 h after both models were established, the decrease in blood flow in the digestive organs occurred earlier than in the brain and kidneys, and the decrease in small intestine blood flow in the LPS group progressed faster.

conclusionsWe have successfully demonstrated the feasibility of our novel method to detect BBB permeability in mice. Our results revealed a significant difference in the BBB permeability change trend between the CLP and LPS model mice when survival curves were consistent. Notably, the CLP-model mice demonstrated a closer resemblance to clinical patients. Our findings suggest that early-stage brain tissue hypoperfusion has a greater impact on BBB function damage in endotoxemia mice, which is related to the faster progression of blood flow redistribution.

Indexed as

Blood-Brain BarrierCerebrovascular CirculationDisease Models, AnimalSepsisAnimalsCapillary PermeabilityEndotoxemiaLipopolysaccharidesMaleMiceMice, Inbred C57BLLipopolysaccharidesBBBCBFIn vivo imagingLaser speckleSepsis

Identifiers

PMID38982003

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.