Evidence map›Paper›PMID 38986360›Full record

ArticleBiomaterials2024

Branching in poly(amine-co-ester) polyplexes impacts mRNA transfection.

Kwangsoo Shin, Hee-Won Suh, Alexandra Suberi, Chang-Hee Whang, Madalina Ene, Julian Grundler, Molly K Grun, W Mark Saltzman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kwangsoo ShinDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA; Department of Polymer Science & Engineering and Program in Environmental and Polymer Engineering, Inha University, Incheon, 22212, Republic of Korea.
Hee-Won SuhDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA.
Alexandra SuberiDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA.
Chang-Hee WhangDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA.
Madalina EneDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA.
Julian GrundlerDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA; Department of Chemistry, Yale University, New Haven, CT, 06511, USA.
Molly K GrunDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA.
W Mark SaltzmanDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06511, USA; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA; Department of Chemical & Environmental Engineering, Yale University, New Haven, CT, 06511, USA; Department of Cellular & Molecular Physiology, Yale School of Medicine, New Haven, CT, 06510, USA; Department of Dermatology, Yale School of Medicine, New Haven, CT, 06510, USA. Electronic address: mark.saltzman@yale.edu.

Funding

Yale Pathology Tissue Services Shared ResourceP30CA016359 · NCI · YALE UNIVERSITY · PI Eric P. Winer · 1985 to 2026
$85.0M
Poly(amine-co-ester)s for Targeted Delivery In Vivo of Gene Editing Agents to Bone Marrow and LungUG3HL147352 · NHLBI · YALE UNIVERSITY · PI GLAZER, PETER M, SALTZMAN, W. MARK · 2018 to 2020
$2.4M
Poly(amine-co-ester)s for targeted delivery of gene editing agents to treat cystic fibrosis in animal models: SCGE Disease Models Studies SupplementUH3HL147352 · NHLBI · YALE UNIVERSITY · PI GLAZER, PETER M, SALTZMAN, W. MARK · 2021 to 2022
$2.4M
PNA Nanoparticles for Gene Editing In VivoU01AI145965 · NIAID · YALE UNIVERSITY · PI GLAZER, PETER M, LY, DANITH H · 2019 to 2022
$1.6M
NCI NIH HHS P30 CA016359NHLBI NIH HHS UG3 HL147352NHLBI NIH HHS UH3 HL147352NIAID NIH HHS U01 AI145965
6 · The paper itself

Abstract

Branching is a key structural parameter of polymers, which can have profound impacts on physicochemical properties. It has been demonstrated that branching is a modulating factor for mRNA delivery and transfection using delivery vehicles built from cationic polymers, but the influence of polymer branching on mRNA delivery remains relatively underexplored compared to other polymer features such as monomer composition, hydrophobicity, pKa, or the type of terminal group. In this study, we examined the impact of branching on the physicochemical properties of poly(amine-co-esters) (PACE) and their efficiency in mRNA transfection in vivo and in vitro under various conditions. PACE polymers were synthesized with various degrees of branching ranging from 0 to 0.66, and their transfection efficiency was systemically evaluated. We observed that branching improves the stability of polyplexes but reduces the pH buffering capacity. Therefore, the degree of branching (DB) must be optimized in a delivery route specific manner due to differences in challenges faced by polyplexes in different physiological compartments. Through a systematic analysis of physicochemical properties and mRNA transfection in vivo and in vitro, this study highlights the influence of polymer branching on nucleic acid delivery.

Indexed as

PolyaminesRNA, MessengerTransfectionAnimalsEstersHumansHydrogen-Ion ConcentrationMicePolymersEstersPolyaminesPolymersRNA, MessengerBranched polymermRNA deliveryNon-viral platformpoly(amino-co-ester)Polyplex

Identifiers

PMID38986360
PMCPMC11298310

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.