Evidence map›Paper›PMID 39008175›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Engineered Heart Tissues for Standard 96-Well Tissue Culture Plates.

Alex J Goldstein, Ruby M Padgett, Samantha B Bremner, Ty Higashi, Ava M Obenaus, Sam Bolduan, David L Mack, Nathan J Sniadecki

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Alex J GoldsteinDepartment of Lab Medicine and Pathology, University of Washington, Seattle, WA, USA.
Ruby M PadgettCenter for Cardiovascular Biology, University of Washington, Seattle, WA, USA.
Samantha B BremnerCenter for Cardiovascular Biology, University of Washington, Seattle, WA, USA.
Ty HigashiCenter for Cardiovascular Biology, University of Washington, Seattle, WA, USA.
Ava M ObenausCenter for Cardiovascular Biology, University of Washington, Seattle, WA, USA.
Sam BolduanDepartment of Mechanical Engineering, University of Washington, Seattle, WA, USA.
David L MackCenter for Cardiovascular Biology, University of Washington, Seattle, WA, USA.
Nathan J SniadeckiDepartment of Lab Medicine and Pathology, University of Washington, Seattle, WA, USA. nsniadec@uw.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Engineered heart tissues (EHTs) have been shown to be a valuable platform for disease investigation and therapeutic testing by increasing human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) maturity and better recreating the native cardiac environment. The protocol detailed in this chapter describes the generation of miniaturized EHTs (mEHTs) incorporating hiPSC-CMs and human stromal cells in a fibrin hydrogel. This platform utilizes an array of silicone posts designed to fit in a standard 96-well tissue culture plate. Stromal cells and hiPSC-CMs are cast in a fibrin matrix suspended between two silicone posts, forming an mEHT that produces synchronous muscle contractions. The platform presented here has the potential to be used for high throughput characterization and screening of disease phenotypes and novel therapeutics through measurements of the myocardial function, including contractile force and calcium handling, and its compatibility with immunostaining.

Indexed as

Induced Pluripotent Stem CellsMyocytes, CardiacTissue EngineeringCell Culture TechniquesCell DifferentiationCells, CulturedFibrinHumansHydrogelsStromal CellsTissue Culture TechniquesFibrinHydrogelsCardiac therapiesCardiac tissue engineeringCardiomyocytesEngineered heart tissueInduced pluripotent stem cells

Identifiers

PMID39008175

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.