Evidence mapPaperPMID 39011037Full record

ArticleFrontiers in immunology2024

Blood immune cell profiling in adults with longstanding type 1 diabetes is associated with macrovascular complications.

Xuehui He, Xinhui Wang, Julia van Heck, Bram van Cranenbroek, Esther van Rijssen, Rinke Stienstra, Mihai G Netea, Irma Joosten, Cees J Tack, Hans J P M Koenen

Abstract read
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Article in Frontiers in immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Xuehui HeDepartment of Laboratory Medicine - Medical Immunology, Radboud University Medical Center, Nijmegen, Netherlands.
Xinhui WangLuxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg.
Julia van HeckDepartment of Internal Medicine, Radboud University Medical Center, Nijmegen, Netherlands.
Bram van CranenbroekDepartment of Laboratory Medicine - Medical Immunology, Radboud University Medical Center, Nijmegen, Netherlands.
Esther van RijssenDepartment of Laboratory Medicine - Medical Immunology, Radboud University Medical Center, Nijmegen, Netherlands.
Rinke StienstraDepartment of Internal Medicine, Radboud University Medical Center, Nijmegen, Netherlands.
Mihai G NeteaDepartment of Internal Medicine, Radboud University Medical Center, Nijmegen, Netherlands.
Irma JoostenDepartment of Laboratory Medicine - Medical Immunology, Radboud University Medical Center, Nijmegen, Netherlands.
Cees J Tack *Department of Internal Medicine, Radboud University Medical Center, Nijmegen, Netherlands.
Hans J P M Koenen *Department of Laboratory Medicine - Medical Immunology, Radboud University Medical Center, Nijmegen, Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aims/hypothesis: There is increasing evidence for heterogeneity in type 1 diabetes mellitus (T1D): not only the age of onset and disease progression rate differ, but also the risk of complications varies markedly. Consequently, the presence of different disease endotypes has been suggested. Impaired T and B cell responses have been established in newly diagnosed diabetes patients. We hypothesized that deciphering the immune cell profile in peripheral blood of adults with longstanding T1D may help to understand disease heterogeneity. Methods: Adult patients with longstanding T1D and healthy controls (HC) were recruited, and their blood immune cell profile was determined using multicolour flow cytometry followed by a machine-learning based elastic-net (EN) classification model. Hierarchical clustering was performed to identify patient-specific immune cell profiles. Results were compared to those obtained in matched healthy control subjects. Results: Hierarchical clustering analysis of flow cytometry data revealed three immune cell composition-based distinct subgroups of individuals: HCs, T1D-group-A and T1D-group-B. In general, T1D patients, as compared to healthy controls, showed a more active immune profile as demonstrated by a higher percentage and absolute number of neutrophils, monocytes, total B cells and activated CD4+CD25+ T cells, while the abundance of regulatory T cells (Treg) was reduced. Patients belonging to T1D-group-A, as compared to T1D-group-B, revealed a more proinflammatory phenotype characterized by a lower percentage of FOXP3+ Treg, higher proportions of CCR4 expressing CD4 and CD8 T cell subsets, monocyte subsets, a lower Treg/conventional Tcell (Tconv) ratio, an increased proinflammatory cytokine (TNFα, IFNγ) and a decreased anti-inflammatory (IL-10) producing potential. Clinically, patients in T1D-group-A had more frequent diabetes-related macrovascular complications. Conclusions: Machine-learning based classification of multiparameter flow cytometry data revealed two distinct immunological profiles in adults with longstanding type 1 diabetes; T1D-group-A and T1D-group-B. T1D-group-A is characterized by a stronger pro-inflammatory profile and is associated with a higher rate of diabetes-related (macro)vascular complications.

Indexed as

Diabetes Mellitus, Type 1AdultCase-Control StudiesDiabetic AngiopathiesFemaleFlow CytometryHumansImmunophenotypingMachine LearningMaleMiddle AgedT-Lymphocytes, Regulatoryheterogeneityhierarchical classificationimmune cell profilemachine learningmultiparameter flow cytometrytype 1 diabetes mellitus

Identifiers

PMID39011037
PMCPMC11246869

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.