Evidence mapPaperPMID 39012819Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2024

AKT-dependent nuclear localization of EPRS1 activates PARP1 in breast cancer cells.

Isaac Zin, Arnab China, Krishnendu Khan, Jeetendra K Nag, Kommireddy Vasu, Gauravi M Deshpande, Prabar K Ghosh, Debjit Khan, Iyappan Ramachandiran, Shinjini Ganguly and 4 more

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Isaac Zin *Department of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.ORCID 0000-0002-9215-9398
Arnab China *Department of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.
Krishnendu KhanDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.
Jeetendra K NagDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.ORCID 0009-0005-1622-6279
Kommireddy VasuDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.
Gauravi M DeshpandeImaging Core, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.ORCID 0000-0002-1621-1553
Prabar K GhoshDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.
Debjit KhanDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.
Iyappan RamachandiranDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.
Shinjini GangulyTranslational Hematology and Oncology Research, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.ORCID 0000-0003-1719-3622
Ilaria TamagnoDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.
Belinda WillardProteomics and Metabolomics Core, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195.ORCID 0000-0001-6884-6369
Valentin GogoneaDepartment of Chemistry, Cleveland State University, Cleveland, OH 44115.ORCID 0000-0002-6154-8497
Paul L FoxDepartment of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195.ORCID 0000-0002-6033-1528

Funding

The mammalian multi-tRNA synthetase complexR01NS124547 · NINDS · CLEVELAND CLINIC LERNER COM-CWRU · 2022 to 2025
$1.9M
Childhood-onset hypomyelinating leukodystrophy and the multi-tRNA synthetase complexR01NS124581 · NINDS · CLEVELAND CLINIC LERNER COM-CWRU · 2024 to 2025
$1.2M
Assay Development for Discovery of a Small Molecule Inhibitor of a Novel Metabolic Pathway that Drives ObesityR01DK124203 · NIDDK · CLEVELAND CLINIC LERNER COM-CWRU · PI PAUL L FOX · 2022 to 2022
$403k
NIA NIH HHS R01 AG067146NIDDK NIH HHS R01 DK123236NIDDK NIH HHS R01 DK124203NINDS NIH HHS R01 NS124547NINDS NIH HHS R01 NS124581
6 · The paper itself

Abstract

Glutamyl-prolyl-tRNA synthetase (EPRS1) is a bifunctional aminoacyl-tRNA-synthetase (aaRS) essential for decoding the genetic code. EPRS1 resides, with seven other aaRSs and three noncatalytic proteins, in the cytoplasmic multi-tRNA synthetase complex (MSC). Multiple MSC-resident aaRSs, including EPRS1, exhibit stimulus-dependent release from the MSC to perform noncanonical activities distinct from their primary function in protein synthesis. Here, we show EPRS1 is present in both cytoplasm and nucleus of breast cancer cells with constitutively low phosphatase and tensin homolog (PTEN) expression. EPRS1 is primarily cytosolic in PTEN-expressing cells, but chemical or genetic inhibition of PTEN, or chemical or stress-mediated activation of its target, AKT, induces EPRS1 nuclear localization. Likewise, preferential nuclear localization of EPRS1 was observed in invasive ductal carcinoma that were also P-Ser

Indexed as

Breast NeoplasmsCell NucleusPoly (ADP-Ribose) Polymerase-1Proto-Oncogene Proteins c-aktActive Transport, Cell NucleusAmino Acyl-tRNA SynthetasesCell Line, TumorFemaleHumansNuclear Localization SignalsPTEN PhosphohydrolaseAmino Acyl-tRNA SynthetasesNuclear Localization SignalsPARP1 protein, humanPoly (ADP-Ribose) Polymerase-1Proto-Oncogene Proteins c-aktPTEN PhosphohydrolasePTEN protein, humanADP-ribosylationAKTaminoacyl-tRNA synthetaseEPRS1PARP1

Identifiers

PMID39012819
PMCPMC11287164

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.